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1.14.99.58: heme oxygenase (biliverdin-IX-beta and delta-forming)

This is an abbreviated version!
For detailed information about heme oxygenase (biliverdin-IX-beta and delta-forming), go to the full flat file.

Reaction

protoheme
+ 3 reduced acceptor + 3 O2 =
biliverdin-IX-beta
+
CO
+
Fe2+
+ 3 acceptor + 3 H2O

Synonyms

biliverdin IXbeta/delta-selective heme oxygenase, BphO, heme-PigA heme oxygenase, heme-PigA(HO), HemO, PigA

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.99 Miscellaneous
                1.14.99.58 heme oxygenase (biliverdin-IX-beta and delta-forming)

Cloned

Cloned on EC 1.14.99.58 - heme oxygenase (biliverdin-IX-beta and delta-forming)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in a Neisseria meningitidis HemO deletion mutant
-
expressed in Escherichia coli BL21(DE3) cells
-
gene bphO, BphO is genetically coupled to the phytochrome BphP, expression of GST-tagged BphO in Escherichia coli strain BL21(DE3)
gene bphO, BphO is genetically coupled to the phytochrome BphP, expression of N-terminally His6-tagged BphO in Escherichia coli strain BL21(DE3)
gene bphOP, DNA and amino acid sequence, including promoter, determination and analysis, expression analysis, dependence of bphOP expression on RpoS
-
gene pigA, pigA genes are organized in gene clusters associated with iron utilization, expression of GST-tagged PigA in Escherichia coli strain BL21(DE3)
genes pigA, two homologues, one of the two PigA homologues identified in Pseudomonas putida KT2440 is encoded in an iron-associated gene cluster, expression of GST-tagged PigA and BphO in Escherichia coli strain BL21(DE3)