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1.14.19.29: sphingolipid 8-(E/Z)-desaturase

This is an abbreviated version!
For detailed information about sphingolipid 8-(E/Z)-desaturase, go to the full flat file.

Reaction

a (4R)-4-hydroxysphinganine ceramide
+ 2 ferrocytochrome b5 +
O2
 + 2 H+ =
a (4R,8E)-4-hydroxysphing-8-enine ceramide
 + 2 ferricytochrome b5 + 2 H2O

Synonyms

8 fatty acid desaturase, 8-sphingolipid desaturase, D8A, D8B, D8C, D8D, DELTA8 Des, Delta8 sphingolipid desaturase, DELTA8(E,Z)-sphingolipid desaturase, Delta8-fatty-acid desaturase 1, Delta8-fatty-acid desaturase 2, DELTA8-sphingolipid desaturase, Delta8-sphingolipid desaturase 1, Delta8-sphingolipid desaturase 2, Delta8-sphingolipid desaturase 3, DELTA8-sphingolipid-desaturase, DES8, DXDES, LCB DELTA8 desaturase, PtD8A, PtD8B, PtD8C, SLD, SLD1, SLD2, sphingoid long-chain base DELTA8 desaturase, sphingolipid DELTA8-desaturase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.19 With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
                1.14.19.29 sphingolipid 8-(E/Z)-desaturase

Cloned

Cloned on EC 1.14.19.29 - sphingolipid 8-(E/Z)-desaturase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed as chimera protein with DELTA6 fatty acid desaturase in Saccharomyces cervisiae
expressed in Saccharomyces cerevisiae
expressed in Saccharomyces cerevisiae and Arabidopsis thaliana
expressed in Saccharomyces cerevisiae BY4741 cells
-
expressed in Saccharomyces cerevisiae strain InvSc1
-
expressed in Saccharomyces cerevisiae strain INVScI
expressed in Saccharomyces cerevisiae strain INVScl mutant sur2DELTA lacking sphinganine C4-hydroxylase
expressed in Saccharomyces cerevisiae strain w303-1a
expressed in Saccharomyces cerevisiae strain W303-A1
expression of this openreading frame in Saccharomyces cerevisiae results in the formation of DELTA8-trans/cis-phytosphingenines not present in wild-type cells
gene AtD8-1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene AtD8-2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene AvD8, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene BdD8, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene BoD8, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene CpD8, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene CsD8, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene GmD8-1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene GmD8-2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene GmD8-3, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene PpD8-1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene PpD8-2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene PpD8-3, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene PtD8-1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene PtD8-2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene PtD8-3, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene PtD8A, sequence comparisons and phylogenetic analysis, semi-quantitative RT-PCR enzyme expression analysis, functional recombinant expression in Saccharomyces cerevisiae strain INVSc1
-
gene PtD8B, sequence comparisons and phylogenetic analysis, semi-quantitative RT-PCR enzyme expression analysis, functional recombinant expression in Saccharomyces cerevisiae strain INVSc1
-
gene RcD8, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene SbD8-1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene TaD8-1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene TaD8-2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene VvD8-1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene VvD8-2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
the open reading frame is cloned behind the constitutive ADH1 promotor of the yeast expression vector pVT-U-102 and transformed in Saccharomyces cerevisiae INVSc1