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Literature summary for 1.14.19.29 extracted from

  • Beckmann Christop, B.C.; Rattke Janin, R.J.; Oldham Neil, O.N.; Sperling Petr, S.P.; Heinz Erns, H.E.; Boland Wilhel, B.W.
    Characterization of a DELTA8-sphingolipid desaturase from higher plants: a stereochemical and mechanistic study on the origin of E,Z isomers (2002), Angew. Chem. Int. Ed. Engl., 41, 2298-2300.
    View publication on PubMed
Search for more literature for 1.14.19.29

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae Helianthus annuus
the open reading frame is cloned behind the constitutive ADH1 promotor of the yeast expression vector pVT-U-102 and transformed in Saccharomyces cerevisiae INVSc1 Helianthus annuus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+ Helianthus annuus
-
 (4R,8E)-4-hydroxysphing-8-enine ceramide + (4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O 7:1 product mixture of (4R,8E)- and (4R,8Z)-4-hydroxy-sphing-8-enine ceramide ?
phytosphinganine + 2 ferrocytochrome b5 + O2 + 2 H+ Helianthus annuus
-
 (4R,8E)-4-hydroxy-8-sphingenine + 2 ferricytochrome b5 + 2 H2O
-
 ?
phytosphinganine + 2 ferrocytochrome b5 + O2 + 2 H+ Helianthus annuus
-
 (4R,8Z)-4-hydroxy-8-sphingenine + 2 ferricytochrome b5 + 2 H2O (2) ?

Organism

Organism UniProt Comment Textmining
Helianthus annuus
-
-
-
Helianthus annuus Q43469
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
-
 Helianthus annuus (4R,8E)-4-hydroxysphing-8-enine ceramide + (4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O 7:1 product mixture of (4R,8E)- and (4R,8Z)-4-hydroxy-sphing-8-enine ceramide ?
phytosphinganine + 2 ferrocytochrome b5 + O2 + 2 H+
-
 Helianthus annuus (4R,8E)-4-hydroxy-8-sphingenine + 2 ferricytochrome b5 + 2 H2O
-
 ?
phytosphinganine + 2 ferrocytochrome b5 + O2 + 2 H+ the enzyme expressed in Saccharomyces cerevisiae converts 4-hydroxy-sphinganine (phytosphinganine) into a 7:1 mixture of (4R,8E)- and (4R,8Z)-4-hydroxy-8-sphingenine. Both desaturation steps leading to either the 8E or the 8Z isomer of 4-hydroxy-8-sphingenine involved the syn elimination of two vicinal hydrogen atoms Helianthus annuus (4R,8E)-4-hydroxy-8-sphingenine + 2 ferricytochrome b5 + 2 H2O
-
 ?
phytosphinganine + 2 ferrocytochrome b5 + O2 + 2 H+
-
 Helianthus annuus (4R,8Z)-4-hydroxy-8-sphingenine + 2 ferricytochrome b5 + 2 H2O (2) ?
phytosphinganine + 2 ferrocytochrome b5 + O2 + 2 H+ the enzyme expressed in Saccharomyces cerevisiae converts 4-hydroxy-sphinganine (phytosphinganine) into a 7:1 mixture of (4R,8E)- and (4R,8Z)-4-hydroxy-8-sphingenine. Both desaturation steps leading to either the 8E or the 8Z isomer of 4-hydroxy-8-sphingenine involved the syn elimination of two vicinal hydrogen atoms Helianthus annuus (4R,8Z)-4-hydroxy-8-sphingenine + 2 ferricytochrome b5 + 2 H2O (2) ?

Synonyms

Synonyms Comment Organism
DELTA8-sphingolipid desaturase
-
 Helianthus annuus

Cofactor

Cofactor Comment Organism Structure
cytochrome b5
-
 Helianthus annuus