1.14.15.6: cholesterol monooxygenase (side-chain-cleaving)

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For detailed information about cholesterol monooxygenase (side-chain-cleaving), go to the full flat file.

Word Map on EC 1.14.15.6

1.14.15.6

steroidogenic

steroidogenesis

star

progesterone

testosterone

ovarian

pregnenolone

granulosa

leydig

ovary

follicle

androgen

testis

testicular

lh

lutein

aromatase

adrenodoxin

gonad

cyp17a1

adrenocortical

estradiol

acth

cortisol

gonadotropin

follicular

3beta-hydroxysteroid

hsd3b1

3beta-hsd

theca

androstenedione

p450arom

aminoglutethimide

cyp11b2

neurosteroids

22r-hydroxycholesterol

21-hydroxylase

lhcgr

hsd17b3

scarb1

synthesis

medicine

cyp21

hcg-stimulated

biotechnology

lipoid

fsh-induced

11beta-hydroxylase

srd5a1

interrenal

analysis

17alpha-hydroxylase

drug development

trilostane

fsh-stimulated

Reaction

+ 2 reduced adrenodoxin +

+ 2 H+ =

+ 2 oxidized adrenodoxin + 2 H2O

Synonyms

C27-side chain cleavage enzyme, cholesterol 20-22-desmolase, cholesterol C20-22 desmolase, cholesterol C20-C22 lyase, cholesterol desmolase, cholesterol hydroxylase, cholesterol side chain cleavage cytochrome P450, cholesterol side chain cleavage enzyme, cholesterol side-chain cleavage cytochrome P450, cholesterol side-chain cleavage cytochrome P450 enzyme, cholesterol side-chain cleavage enzyme, cholesterol side-chain-cleaving enzyme, cholesterol side-cleaving enzyme, CYP 11A1, Cyp11a, CYP11A1, CYPXIA1, cytochrome P-450scc, cytochrome P450 11A1, cytochrome P450 cholesterol side chain cleavage, cytochrome P450 cholesterol side-chain cleavage, cytochrome P450 side chain cleavage enzyme, cytochrome P450-mediated cholesterol side-chain cleavage enzyme, cytochrome P450-mediated side-chain cleavage enzyme, cytochrome P450scc, desmolase, steroid 20-22, endoenzymes, cholesterol side-chain-cleaving, enzymes, cholesterol side-chain-cleaving, P450 11A1, P450 cholesterol side chain cleaving enzyme, P450 cholesterol side-chain cleavage enzyme, P450(scc), P450scc, steroid 20-22 desmolase, steroid 20-22-lyase

ECTree

1 Oxidoreductases

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.14.15 With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor

1.14.15.6 cholesterol monooxygenase (side-chain-cleaving)

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Results	in table
26	Activating Compound
1312	AA Sequence
7	Application
1	CAS Registry Number
21	Cloned(Commentary)
85	Cofactor
2	Crystallization (Commentary)
814	Disease/ Diagnostics
12	Expression
13	General Information
2	General Stability
35	Inhibitors
5	kcat/KM [mM/s]
23	KM Value [mM]
50	Localization
15	Metals/Ions
35	Molecular Weight [Da]
43	Natural Substrates/ Products (Substrates)
1	Organic Solvent Stability
236	Organism
4	Pathway
12	PDB ID
19	pH Optimum
1	pH Range
38	Protein Variants
31	Purification (Commentary)
4	Reaction
3	Reaction Type
70	Reference
104	Source Tissue
11	Storage Stability
121	Substrates and Products (Substrate)
13	Subunits
96	Synonyms
1	Systematic Name
16	Temperature Optimum [°C]
1	Temperature Range [°C]
1	Temperature Stability [°C]
10	Turnover Number [1/s]

Molecular Weight

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Molecular Weight on EC 1.14.15.6 - cholesterol monooxygenase (side-chain-cleaving)

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100000

Bos taurus

native-PAGE, incomplete enzyme or molecular environmental conditions not optimal

120000

200000

220000

corpus luteum

225000

sedimentation equilibrium

32000

400000

native-PAGE

415000

sedimentation equilibrium

285423

46000

3 entries

470000

Bos taurus

sedimentation equilibrium performed in 100 mM potassium phosphate buffer, pH 7.6

285427

48000

Bos taurus

2,4,6,8 * 48000, SDS-PAGE, corpus luteum. x * 46000, x * 60000, x * 53000, x * 52000-53000 adrenal mitochondria

285423

51400

Mytilus galloprovincialis

x * 51400, SDS-PAGE

52000

53000

55000

59000

x * 59000, recombinant His-tagged enzyme, SDS-PAGE

673821

60000

2 entries

800000

Bos taurus

exclusion chromatography

285418

850000

3 entries

97000

Bos taurus

sedimentation equilibrium

285423