Literature summary for 1.14.15.6 extracted from

Teplyuk, N.M.; Zhang, Y.; Lou, Y.; Hawse, J.R.; Hassan, M.Q.; Teplyuk, V.I.; Pratap, J.; Galindo, M.; Stein, J.L.; Stein, G.S.; Lian, J.B.; van Wijnen, A.J.
The osteogenic transcription factor runx2 controls genes involved in sterol/steroid metabolism, including CYP11A1 in osteoblasts (2009), Mol. Endocrinol., 23, 849-861.

Search for more literature for 1.14.15.6

Activating Compound

Activating Compound	Comment	Organism	Structure
Runx2	activation of CYP11A1 by the osteogenic transcription factor Runx2 may contribute to attenuation of osteoblast growth. Runx2 binds to the CYP11A1 gene promoter, CYP11A1 is a direct target of Runx2	Homo sapiens
Runx2	activation of CYP11A1 by the osteogenic transcription factor Runx2 may contribute to attenuation of osteoblast growth. Runx2 binds to the CYP11A1 gene promoter, CYP11A1 is a direct target of Runx2. Runx2 is rate limiting for CYP11A1 gene expression during differentation along the osteoblastic lineage	Mus musculus

Cloned(Commentary)

Cloned (Comment)	Organism
both full-length and an N-terminally truncated form amplified and inserted into TOPO TA PCR cloning vector and subsequently subcloned into the EcoRI restriction site of pCMV-tag2B vector to obtain CYP11A1 proteins with an N-terminal Flag tag or into pEGFP-C2 vector to yield an N-terminal GFP tag	Mus musculus
expressed from pCMV-SPORT6 vector	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	both full length and truncated isoforms are predominantely localized in the cytoplasm	Mus musculus	5737	-
mitochondrion	truncated form is nonmitochondrial	Mus musculus	5739	-
nucleus	truncated form	Mus musculus	5634	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
32000	-	truncated form, Western blot analysis	Mus musculus
32000	-	truncated form, Western blot analysis	Homo sapiens
55000	-	full-length form, Western blot analysis	Mus musculus
55000	-	full-length form, Western blot analysis	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-
Mus musculus	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
calvarium	low level	Mus musculus	-
MDA-MB-231 cell	breast cancer cell, expresses both the 55-kDa isoform and the 32-kDa truncated isoform of CYP11A1	Homo sapiens	-
additional information	expression of the 32-kDa truncated isoform of CYP11A1 in HOB-04T8 cells	Homo sapiens	-
additional information	very low level of endogenous CYP11A1 in MC3T3 cells and Runx2-null osteoprogenitor cells	Mus musculus	-
osteoblast	-	Homo sapiens	-
osteoblast	expresses an approximately 32-kDa truncated isoform of CYP11A1, expression of an approximately 32-kDa truncated isoform of CYP11A1	Mus musculus	-
ovary	high levels in mature tissue	Mus musculus	-
prostate	expresses the full length 55-kDa isoform of CYP11A1	Mus musculus	-
testis	high level, expresses the full length 55-kDa isoform of CYP11A1	Mus musculus	-
tibia	low level	Mus musculus	-

Synonyms

Synonyms	Comment	Organism
cholesterol side-cleaving enzyme	-	Mus musculus
cholesterol side-cleaving enzyme	-	Homo sapiens
CYP11A1	-	Mus musculus
CYP11A1	-	Homo sapiens
P450scc	-	Mus musculus
P450scc	-	Homo sapiens

Expression

Organism	Comment	Expression
Homo sapiens	CYP11A1 expression is induced by Runx2in osteoblasts	up
Mus musculus	CYP11A1 mRNA expression is dramatically induced within 2 h after introduction of Runx2 protein into Runx2-null cells. CYP11A1 gene expression is induced during osteoblastic differentiation	up

General Information

General Information	Comment	Organism
metabolism	CYP11A1 is a Runx2-responsive gene and may represent an integral component of a biological program that is controlled by Runx2 to regulate enzymes mediating sterol/steroid metabolism	Mus musculus
metabolism	CYP11A1 is a Runx2-responsive gene and may represent an integral component of a biological program that is controlled by Runx2 to regulate enzymes mediating sterol/steroid metabolism	Homo sapiens
physiological function	CYP11A1 suppresses osteoblast proliferation, specific CYP11A1 knockdown with short hairpin RNA increases cell proliferation	Mus musculus

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Release 2023.1 (January 2023)