1.14.15.6: cholesterol monooxygenase (side-chain-cleaving)

This is an abbreviated version!
For detailed information about cholesterol monooxygenase (side-chain-cleaving), go to the full flat file.

Reaction

Synonyms

C27-side chain cleavage enzyme, cholesterol 20-22-desmolase, cholesterol C20-22 desmolase, cholesterol C20-C22 lyase, cholesterol desmolase, cholesterol hydroxylase, cholesterol side chain cleavage cytochrome P450, cholesterol side chain cleavage enzyme, cholesterol side-chain cleavage cytochrome P450, cholesterol side-chain cleavage cytochrome P450 enzyme, cholesterol side-chain cleavage enzyme, cholesterol side-chain-cleaving enzyme, cholesterol side-cleaving enzyme, CYP 11A1, Cyp11a, CYP11A1, CYPXIA1, cytochrome P-450scc, cytochrome P450 11A1, cytochrome P450 cholesterol side chain cleavage, cytochrome P450 cholesterol side-chain cleavage, cytochrome P450 side chain cleavage enzyme, cytochrome P450-mediated cholesterol side-chain cleavage enzyme, cytochrome P450-mediated side-chain cleavage enzyme, cytochrome P450scc, desmolase, steroid 20-22, endoenzymes, cholesterol side-chain-cleaving, enzymes, cholesterol side-chain-cleaving, P450 11A1, P450 cholesterol side chain cleaving enzyme, P450 cholesterol side-chain cleavage enzyme, P450(scc), P450scc, steroid 20-22 desmolase, steroid 20-22-lyase

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.15 With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor

                1.14.15.6 cholesterol monooxygenase (side-chain-cleaving)

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
26	Activating Compound
1312	AA Sequence
7	Application
1	CAS Registry Number
21	Cloned(Commentary)
85	Cofactor
2	Crystallization (Commentary)
814	Disease/ Diagnostics
12	Expression
13	General Information
2	General Stability
35	Inhibitors
5	kcat/KM [mM/s]
23	KM Value [mM]
50	Localization
15	Metals/Ions
35	Molecular Weight [Da]
43	Natural Substrates/ Products (Substrates)
1	Organic Solvent Stability
236	Organism
4	Pathway
12	PDB ID
19	pH Optimum
1	pH Range
38	Protein Variants
31	Purification (Commentary)
4	Reaction
3	Reaction Type
70	Reference
104	Source Tissue
11	Storage Stability
121	Substrates and Products (Substrate)
13	Subunits
96	Synonyms
1	Systematic Name
16	Temperature Optimum [°C]
1	Temperature Range [°C]
1	Temperature Stability [°C]
10	Turnover Number [1/s]

General Information

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

General Information on EC 1.14.15.6 - cholesterol monooxygenase (side-chain-cleaving)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide show all columns Go to General Information Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

GENERAL INFORMATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

metabolism

5 entries

physiological function

7 entries

additional information

Bos taurus

P00189

the active site cavity in CYP11A1 represents a long curved tube that extends from the protein surface to the heme group, the site of catalysis. Shuttling of the sterol intermediates between the active site entrance and the heme group during the three-step reaction. Structural basis of the strict substrate specificity and high catalytic efficiency of the enzyme, overview

725459

metabolism

Salmo salar

-

conversion of cholesterol to pregnenolone by P450scc represents the initiation of steroidogenesis

703100

metabolism

Mus musculus

-

CYP11A1 is a Runx2-responsive gene and may represent an integral component of a biological program that is controlled by Runx2 to regulate enzymes mediating sterol/steroid metabolism

705739

metabolism

Homo sapiens

-

CYP11A1 is a Runx2-responsive gene and may represent an integral component of a biological program that is controlled by Runx2 to regulate enzymes mediating sterol/steroid metabolism

705739

metabolism

Bos taurus

-

vitamin D2 metabolism occurs with lower efficiency than that observed for both cholesterol and D3 metabolism by P450scc

703418

metabolism

Oryzias latipes

-

Cyp11a is responsible for the first step in steroidogenesis, catalyzing the conversion of cholesterol to prognenolone

711826

physiological function

Mus musculus

-

CYP11A1 suppresses osteoblast proliferation, specific CYP11A1 knockdown with short hairpin RNA increases cell proliferation

705739

physiological function

Gadus morhua

-

endocrine disrupting chemical modulation of acute steroid hormone regulation by targeting StAR and P450scc may have severe consequences for the entire reproduction process, including oocyte growth, development and overt fecundity

703100

physiological function

Bos taurus

-

Escherichia coli membrane interacting peptides in CYP11A1 are from the putative F-G loop (residues 218-225) and the C-terminal portion of the G helix (residues 238-250)

705461

physiological function

Oncorhynchus mykiss

-

expression of StAR and P450scc transcripts is related to steroid synthesis

703100

physiological function

Homo sapiens

-

cytochrome P450scc metabolizes vitamin D3 to 20-hydroxyvitamin D3 and 20,23-dihydroxyvitamin D3, as well as 1-hydroxyvitamin D3 to 1alpha,20-dihydroxyvitamin D3. It also cleaves the side chain of 7-dehydrocholesterol producing 7-dehydropregnenolone, which can be transformed to 20-hydroxy-7-dehydropregnenolone. The secosteroids inhibit proliferation and induce erythroid differentiation of diverse leukemic cells, and are only slightly less potent than 1,25-dihydroxy-vitamin D3, but show no or lower calcemic activity compared to 1,25-dihydroxy-vitamin D3, molecular modeling, overview

713379

physiological function

Hypanus sabinus

D0EFX3

the enzyme shows steroidogenic activity. The stress peptide adrenocorticotropic hormone, ACTH, elicits its effects on corticosteroidogenesis in part through regulation of StAR and P450scc mRNAs. Chronic ACTH-induced glucocorticoid synthesis in elasmobranchs is mediated by regulation of primary steroidogenic mRNAs

712096

physiological function

Bos taurus

P00189

the activity of P450scc is dependent upon electron delivery from NADPH-dependent adrenodoxin reductase (AdR), via adrenodoxin (Adx). The lipid membrane assists in the assembly of electron transport proteins and the activity of P450scc by providing a surface for the localized concentration of proteins. Using sequential deposition with P450scc first (to saturation), there is effectively no further binding of AdR or Adx. When premixing the three proteins, P450scc, Adx and AdR, all bind the membrane in a two-stage process

744728