1.14.13.114: 6-hydroxynicotinate 3-monooxygenase
This is an abbreviated version!
For detailed information about 6-hydroxynicotinate 3-monooxygenase, go to the full flat file.
Reaction
Synonyms
6-hydroxynicotinic acid 3-monooxygenase, 6HNA monooxygenase, BB1770, BbNicC, NicC, PpNicC
ECTree
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KM Value
KM Value on EC 1.14.13.114 - 6-hydroxynicotinate 3-monooxygenase
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0.0039
5-chloro-6-hydroxynicotinate
pH 7.5, 25°C, recombinant wild-type enzyme
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0.6
4-hydroxybenzoate
pH 7.5, 25°C, recombinant wild-type enzyme
0.02
pH 7.5, 25°C, recombinant mutant H302A
0.051
6-Hydroxynicotinate
pH 7.5, 25°C, recombinant mutant Y225F
0.118
6-Hydroxynicotinate
pH 7.5, 25°C, recombinant wild-type enzyme
0.193
6-Hydroxynicotinate
pH 7.5, 25°C, recombinant mutant H211A
0.2
6-Hydroxynicotinate
pH 7.5, 25°C, recombinant mutant C202A
1.6
6-Hydroxynicotinate
pH 7.5, 25°C, recombinant mutant Y215F
additional information
additional information
kinetic analysis
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additional information
additional information
Michaelis-Menten kinetics, comparative steady-state kinetic analysis with different substrates, 13C kinetic isotope effects, wild-type enzyme and mutants, overview. Comparison of the initial steady-state rates of product (2,5-DHP and NAD+) formation measured by HPLC and equilibrium dissociation constants for the NicC-FAD-6HNA complex
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