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1.14.13.114: 6-hydroxynicotinate 3-monooxygenase

This is an abbreviated version!
For detailed information about 6-hydroxynicotinate 3-monooxygenase, go to the full flat file.

Reaction

6-Hydroxynicotinate
+
NADH
+
H+
+
O2
=
2,5-dihydroxypyridine
+
NAD+
+
H2O
+
CO2

Synonyms

6-hydroxynicotinic acid 3-monooxygenase, 6HNA monooxygenase, BB1770, BbNicC, NicC, PpNicC

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.114 6-hydroxynicotinate 3-monooxygenase

Purification

Purification on EC 1.14.13.114 - 6-hydroxynicotinate 3-monooxygenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography