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1.14.11.53: mRNA N6-methyladenine demethylase

This is an abbreviated version!
For detailed information about mRNA N6-methyladenine demethylase, go to the full flat file.

Word Map on EC 1.14.11.53

Reaction

N6-methyladenine in mRNA
+
2-oxoglutarate
 +
O2
=
adenine in mRNA
 +
formaldehyde
 +
succinate
 +
CO2

Synonyms

AlkB homolog 5, ALKBH10B, ALKBH5, ALKBH5 demethylase, ALKBH9B, alkylation repair homolog protein 5, fat mass and obesity-associated enzyme, fat mass and obesity-associated protein, FTO, m6A mRNA demethylase, m6A RNA demethylase, m6A-RNA demethylase, N6-methyladenosine demethylase, RNA N6-methyladenine demethylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
                1.14.11.53 mRNA N6-methyladenine demethylase

Engineering

Engineering on EC 1.14.11.53 - mRNA N6-methyladenine demethylase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
269A/Q271A
double mutation shows no impact on the repair efficiency of Alkbh5
E153A
mutant enzyme displays activity similar to wild type
F232A/F234A
mutant exhibits 41% activity toward m6A-containing ssDNA
F232D/Q233D/F234E
the mutant enzyme displays a severe loss of activity, demonstrating only 13.5% of wild-type activity
H204A
inactive mutant enzyme
H231A/D233A
H266A
activity is significantly compromised
I209D
-
the mutant shows decreased activity compared to the wild type enzyme
I209E
-
the mutant shows decreased activity compared to the wild type enzyme
K132Q
mutant furnishes the ALKBH5 enzyme with an m6A demethylation profile that resembles that of isoform FTO
K231A/K235A
double mutation shows no impact on the repair capacity of Alkbh5
K231E/K235E
double mutation shows no impact on the repair capacity of Alkbh5
K86R/K321R
ROS-induced global mRNA m6A modification is completely blocked by mutant K86R/K321R overexpression
Q146A/K147A/R148A
mutant retains 44% of the wild-type activity
R130A
no detectable activity toward m6A RNA
R269E/Q271E
mutant with greatly reduced catalytic activity to 50%
R316Q
mutation abolishes 80% of the wild-type activity towards m6A demethylation in vitro
R316Q/R322Q
mutant completely loses m6A demethylation activity
Y139A
no detectable activity toward m6A RNA
additional information
residues located between 387 and 427 are critical for the interaction with the alfalfa mosaic virus coat protein, which should be critical for modulating the viral infection process. ALKBH9B deletions of either N-terminal 20 residues or the C-terminal's last 40 amino acids impede their accumulation in siRNA bodies