Protein Variants | Comment | Organism |
---|---|---|
H231A/D233A | mutant completely loses m6A demethylation activity | Homo sapiens |
R316Q | mutation abolishes 80% of the wild-type activity towards m6A demethylation in vitro | Homo sapiens |
R316Q/R322Q | mutant completely loses m6A demethylation activity | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | localised in nuclear speckles, which are subnuclear structures that are enriched in pre-messenger RNA splicing factors | Homo sapiens | 5634 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N6-methyladenine in mRNA + 2-oxoglutarate + O2 | Homo sapiens | m6A in nuclear RNA is the physiological substrate of the enzyme | adenine in mRNA + formaldehyde + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9C0B1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | negligible activity with m6A-containing dsDNA and dsRNA. Treatment with one molar equivalent of enzyme at 16°C overnight results in a demethylation yield of 40% for dsDNA and 24% for dsRNA, respectively | Homo sapiens | ? | - |
? | |
N6-methyladenine in mRNA + 2-oxoglutarate + O2 | m6A in nuclear RNA is the physiological substrate of the enzyme | Homo sapiens | adenine in mRNA + formaldehyde + succinate + CO2 | - |
? | |
N6-methyladenine in mRNA + 2-oxoglutarate + O2 | 15-mer m6A-containing ssRNA or 15-mer m3U-containing ssRNA. Over 50-fold preference of the enzyme for m6A (at pH 7.0) over m3U (qat pH 6.0) | Homo sapiens | adenine in mRNA + formaldehyde + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
fat mass and obesity-associated protein | - |
Homo sapiens |
FTO | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Homo sapiens |