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Literature summary for 1.14.11.53 extracted from
- N6-methyladenosine in nuclear RNA is a major substrate of the obesity-associated FTO (2011), Nat. Chem. Biol., 7, 885-887.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H231A/D233A | mutant completely loses m6A demethylation activity | Homo sapiens |
| R316Q | mutation abolishes 80% of the wild-type activity towards m6A demethylation in vitro | Homo sapiens |
| R316Q/R322Q | mutant completely loses m6A demethylation activity | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| nucleus | localised in nuclear speckles, which are subnuclear structures that are enriched in pre-messenger RNA splicing factors | Homo sapiens | 5634 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N6-methyladenine in mRNA + 2-oxoglutarate + O2 | Homo sapiens | m6A in nuclear RNA is the physiological substrate of the enzyme | adenine in mRNA + formaldehyde + succinate + CO2 | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q9C0B1 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | negligible activity with m6A-containing dsDNA and dsRNA. Treatment with one molar equivalent of enzyme at 16°C overnight results in a demethylation yield of 40% for dsDNA and 24% for dsRNA, respectively | Homo sapiens | ? | - |
? | |
| N6-methyladenine in mRNA + 2-oxoglutarate + O2 | m6A in nuclear RNA is the physiological substrate of the enzyme | Homo sapiens | adenine in mRNA + formaldehyde + succinate + CO2 | - |
? | |
| N6-methyladenine in mRNA + 2-oxoglutarate + O2 | 15-mer m6A-containing ssRNA or 15-mer m3U-containing ssRNA. Over 50-fold preference of the enzyme for m6A (at pH 7.0) over m3U (qat pH 6.0) | Homo sapiens | adenine in mRNA + formaldehyde + succinate + CO2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| fat mass and obesity-associated protein | - |
Homo sapiens |
| FTO | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Homo sapiens |
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Release 2023.1 (January 2023)
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