1.1.1.B19: xylitol dehydrogenase (NAD+)

This is an abbreviated version, for detailed information about xylitol dehydrogenase (NAD+), go to the full flat file.

Reaction

xylitol
+
NAD+
=
L-xylulose
+
NADH
+
H+

Synonyms

NAD+-linked xylitol dehydrogenase, NADþ-dependent XDH, XDH, XYL2, xylitol dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.B19 xylitol dehydrogenase (NAD+)

Cloned

Cloned on EC 1.1.1.B19 - xylitol dehydrogenase (NAD+)

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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
gene XYL2, recombinant expression in Saccharomyces cerevisiae strain SCB7 resulting in strains SCF202 and SCF201, coexpression of XYL2 with XYL1, encoding a xylose reductase, and the endogenous XKS1, encoding a xylulokinase, all under the control of the TDH3 promoter in the chromosomal DNA, the recombinant strain efficiently grows in minimal medium containing xylose as the sole carbon source. An additional mutation of MTH1, encoding the endogenous negative regulator of the glucose-sensing signal transduction pathway, MTH1e32, and GRR1, encoding endogenous Grr1, an Fbox protein component of an SCF ubiquitineligase complex grr1e33, enable efficient growth in medium containing high xylose concentrations, subcloning in Escherichia coli strain DH10B
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