Information on EC 1.1.1.B19 - xylitol dehydrogenase (NAD+)

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The expected taxonomic range for this enzyme is: Aeribacillus pallidus

EC NUMBER
COMMENTARY hide
1.1.1.B19
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
xylitol dehydrogenase (NAD+)
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
xylitol + NAD+ = L-xylulose + NADH + H+
show the reaction diagram
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
xylitol:NAD+ 2-oxidoreductase (L-xylulose-forming)
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-arabitol + NADH + H+
D-ribulose + NAD+
show the reaction diagram
15% conversion in resting cell reaction
-
-
?
D-iditol + NAD+
D-sorbose + NADH + H+
show the reaction diagram
72% conversion in resting cell reaction
-
-
?
D-talitol + NADH + H+
D-tagatose + NAD+
show the reaction diagram
15% conversion in resting cell reaction
-
-
?
D-threitol + NAD+
erythrulose + NADH + H+
show the reaction diagram
48% conversion in resting cell reaction
-
-
?
D-xylulose + NADH + H+
xylitol + NAD+
show the reaction diagram
-
-
-
?
ribitol + NADH + H+
D-ribulose + NAD+
show the reaction diagram
15% conversion in resting cell reaction
-
-
?
xylitol + NAD+
L-xylulose + NADH + H+
show the reaction diagram
50% conversion in resting cell reaction
-
-
r
additional information
?
-
no substrate: DL-rhamnitol, galactitol, allitol, DL-fucitol, L-iditol, L-threitol, DL-sorbitol, DL-mannitol, erythritol
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NAD+
specific for NAD+
additional information
no activity with NADP+
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
1 mM, 50.8% of the activity with Mn2+
Mg2+
1 mM, 36.9% of the activity with Mn2+
additional information
no significant stimulation by Na+, Cu2+, K+, Zn2+, Co2+
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.64
D-arabitol
pH 10.0, 40°C
0.83
D-threitol
pH 10.0, 40°C
1.27
L-xylulose
pH 10.0, 40°C
0.034
NAD+
pH 10.0, 40°C
2.06
xylitol
pH 10.0, 40°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.695
D-arabitol
Aeribacillus pallidus
E0D583
pH 10.0, 40°C
2.75
D-threitol
Aeribacillus pallidus
E0D583
pH 10.0, 40°C
1.11
L-xylulose
Aeribacillus pallidus
E0D583
pH 10.0, 40°C
2.75
xylitol
Aeribacillus pallidus
E0D583
pH 10.0, 40°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.26
D-arabitol
Aeribacillus pallidus
E0D583
pH 10.0, 40°C
1786
3.31
D-threitol
Aeribacillus pallidus
E0D583
pH 10.0, 40°C
3460
0.87
L-xylulose
Aeribacillus pallidus
E0D583
pH 10.0, 40°C
1238
1.33
xylitol
Aeribacillus pallidus
E0D583
pH 10.0, 40°C
416
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
27333
x * 27333, calculated, x * 28000, SDS-PAGE
28000
x * 27333, calculated, x * 28000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 27333, calculated, x * 28000, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
1 h, 66% residual acitivity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
production of L-xylulose from xylitol using a resting cell reaction leads to 35% L-xylulose within 24 h, starting from 5% xylitol as initial concentration