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show all sequences of 1.1.1.B19

Cloning and overexpression of the xylitol dehydrogenase gene from Bacillus pallidus and its application to L-xylulose production

Takata, G.; Poonperm, W.; Morimoto, K.; Izumori, K.; Biosci. Biotechnol. Biochem. 74, 1807-1813 (2010)

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
production of L-xylulose from xylitol using a resting cell reaction leads to 35% L-xylulose within 24 h, starting from 5% xylitol as initial concentration
Aeribacillus pallidus
Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Aeribacillus pallidus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.034
-
NAD+
pH 10.0, 40°C
Aeribacillus pallidus
0.83
-
D-threitol
pH 10.0, 40°C
Aeribacillus pallidus
1.27
-
L-xylulose
pH 10.0, 40°C
Aeribacillus pallidus
2.06
-
xylitol
pH 10.0, 40°C
Aeribacillus pallidus
2.64
-
D-arabitol
pH 10.0, 40°C
Aeribacillus pallidus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
1 mM, 50.8% of the activity with Mn2+
Aeribacillus pallidus
Mg2+
1 mM, 36.9% of the activity with Mn2+
Aeribacillus pallidus
Mn2+
required
Aeribacillus pallidus
additional information
no significant stimulation by Na+, Cu2+, K+, Zn2+, Co2+
Aeribacillus pallidus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27333
-
x * 27333, calculated, x * 28000, SDS-PAGE
Aeribacillus pallidus
28000
-
x * 27333, calculated, x * 28000, SDS-PAGE
Aeribacillus pallidus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aeribacillus pallidus
E0D583
-
-
Purification (Commentary)
Commentary
Organism
recombinant enzyme
Aeribacillus pallidus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-arabitol + NADH + H+
15% conversion in resting cell reaction
711517
Aeribacillus pallidus
D-ribulose + NAD+
-
-
-
?
D-iditol + NAD+
72% conversion in resting cell reaction
711517
Aeribacillus pallidus
D-sorbose + NADH + H+
-
-
-
?
D-talitol + NADH + H+
15% conversion in resting cell reaction
711517
Aeribacillus pallidus
D-tagatose + NAD+
-
-
-
?
D-threitol + NAD+
48% conversion in resting cell reaction
711517
Aeribacillus pallidus
erythrulose + NADH + H+
-
-
-
?
D-xylulose + NADH + H+
-
711517
Aeribacillus pallidus
xylitol + NAD+
-
-
-
?
additional information
no substrate: DL-rhamnitol, galactitol, allitol, DL-fucitol, L-iditol, L-threitol, DL-sorbitol, DL-mannitol, erythritol
711517
Aeribacillus pallidus
?
-
-
-
-
ribitol + NADH + H+
15% conversion in resting cell reaction
711517
Aeribacillus pallidus
D-ribulose + NAD+
-
-
-
?
xylitol + NAD+
50% conversion in resting cell reaction
711517
Aeribacillus pallidus
L-xylulose + NADH + H+
-
-
-
r
Subunits
Subunits
Commentary
Organism
?
x * 27333, calculated, x * 28000, SDS-PAGE
Aeribacillus pallidus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
55
-
-
Aeribacillus pallidus
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
1 h, 66% residual acitivity
Aeribacillus pallidus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.695
-
D-arabitol
pH 10.0, 40°C
Aeribacillus pallidus
1.11
-
L-xylulose
pH 10.0, 40°C
Aeribacillus pallidus
2.75
-
D-threitol
pH 10.0, 40°C
Aeribacillus pallidus
2.75
-
xylitol
pH 10.0, 40°C
Aeribacillus pallidus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
11
-
-
Aeribacillus pallidus
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no activity with NADP+
Aeribacillus pallidus
NAD+
specific for NAD+
Aeribacillus pallidus
Application (protein specific)
Application
Commentary
Organism
synthesis
production of L-xylulose from xylitol using a resting cell reaction leads to 35% L-xylulose within 24 h, starting from 5% xylitol as initial concentration
Aeribacillus pallidus
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Aeribacillus pallidus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no activity with NADP+
Aeribacillus pallidus
NAD+
specific for NAD+
Aeribacillus pallidus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.034
-
NAD+
pH 10.0, 40°C
Aeribacillus pallidus
0.83
-
D-threitol
pH 10.0, 40°C
Aeribacillus pallidus
1.27
-
L-xylulose
pH 10.0, 40°C
Aeribacillus pallidus
2.06
-
xylitol
pH 10.0, 40°C
Aeribacillus pallidus
2.64
-
D-arabitol
pH 10.0, 40°C
Aeribacillus pallidus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
1 mM, 50.8% of the activity with Mn2+
Aeribacillus pallidus
Mg2+
1 mM, 36.9% of the activity with Mn2+
Aeribacillus pallidus
Mn2+
required
Aeribacillus pallidus
additional information
no significant stimulation by Na+, Cu2+, K+, Zn2+, Co2+
Aeribacillus pallidus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27333
-
x * 27333, calculated, x * 28000, SDS-PAGE
Aeribacillus pallidus
28000
-
x * 27333, calculated, x * 28000, SDS-PAGE
Aeribacillus pallidus
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant enzyme
Aeribacillus pallidus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-arabitol + NADH + H+
15% conversion in resting cell reaction
711517
Aeribacillus pallidus
D-ribulose + NAD+
-
-
-
?
D-iditol + NAD+
72% conversion in resting cell reaction
711517
Aeribacillus pallidus
D-sorbose + NADH + H+
-
-
-
?
D-talitol + NADH + H+
15% conversion in resting cell reaction
711517
Aeribacillus pallidus
D-tagatose + NAD+
-
-
-
?
D-threitol + NAD+
48% conversion in resting cell reaction
711517
Aeribacillus pallidus
erythrulose + NADH + H+
-
-
-
?
D-xylulose + NADH + H+
-
711517
Aeribacillus pallidus
xylitol + NAD+
-
-
-
?
additional information
no substrate: DL-rhamnitol, galactitol, allitol, DL-fucitol, L-iditol, L-threitol, DL-sorbitol, DL-mannitol, erythritol
711517
Aeribacillus pallidus
?
-
-
-
-
ribitol + NADH + H+
15% conversion in resting cell reaction
711517
Aeribacillus pallidus
D-ribulose + NAD+
-
-
-
?
xylitol + NAD+
50% conversion in resting cell reaction
711517
Aeribacillus pallidus
L-xylulose + NADH + H+
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 27333, calculated, x * 28000, SDS-PAGE
Aeribacillus pallidus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
55
-
-
Aeribacillus pallidus
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
1 h, 66% residual acitivity
Aeribacillus pallidus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.695
-
D-arabitol
pH 10.0, 40°C
Aeribacillus pallidus
1.11
-
L-xylulose
pH 10.0, 40°C
Aeribacillus pallidus
2.75
-
D-threitol
pH 10.0, 40°C
Aeribacillus pallidus
2.75
-
xylitol
pH 10.0, 40°C
Aeribacillus pallidus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
11
-
-
Aeribacillus pallidus
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.26
-
D-arabitol
pH 10.0, 40°C
Aeribacillus pallidus
0.87
-
L-xylulose
pH 10.0, 40°C
Aeribacillus pallidus
1.33
-
xylitol
pH 10.0, 40°C
Aeribacillus pallidus
3.31
-
D-threitol
pH 10.0, 40°C
Aeribacillus pallidus
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.26
-
D-arabitol
pH 10.0, 40°C
Aeribacillus pallidus
0.87
-
L-xylulose
pH 10.0, 40°C
Aeribacillus pallidus
1.33
-
xylitol
pH 10.0, 40°C
Aeribacillus pallidus
3.31
-
D-threitol
pH 10.0, 40°C
Aeribacillus pallidus
Other publictions for EC 1.1.1.B19
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
711517
Takata
Cloning and overexpression of ...
Aeribacillus pallidus
Biosci. Biotechnol. Biochem.
74
1807-1813
2010
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