1.1.1.82: malate dehydrogenase (NADP+)

This is an abbreviated version, for detailed information about malate dehydrogenase (NADP+), go to the full flat file.

Reaction

(S)-malate
+
NADP+
=
oxaloacetate
+
NADPH
+
H+

Synonyms

(S)-malate dehydrogenase, dehydrogenase, malate (nicotinamide adenine dinucleotide phosphate), malate NADP dehydrogenase, malic dehydrogenase (nicotinamide adenine dinucleotide phosphate), MDH, NADP malate dehydrogenase, NADP-dependent malate dehydrogenase, NADP-linked malate dehydrogenase, NADP-malate dehydrogenase, NADP-malic enzyme, NADP-MDH, NADP-MDH1, NADP-MDH2, NADPH-MDH

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.82 malate dehydrogenase (NADP+)

Cloned

Cloned on EC 1.1.1.82 - malate dehydrogenase (NADP+)

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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli, expression vectors pJ2PCC1 and pJ2PCC2
-
expression of MDH in Escherichia coli strain BL21(DE3) in inclusion bodies
high-level expression in Escherichia coli, wild-type enzyme and mutant enzymes C64S, C69S, C64S/C69S and DELTAN, a truncation mutant corresponding to the deletion of the 5' end of the mdh cDNA open reading frame until the 73rd codon
Sorghum sp.
-
structure and characterization of the gene, the gene contains 13 introns and 14 exons which encode a 429 amino acid precursor. The gene transcript contains an untranslated leader sequence of 44 nucleotides
-