1.1.1.82: malate dehydrogenase (NADP+)

This is an abbreviated version, for detailed information about malate dehydrogenase (NADP+), go to the full flat file.

Reaction

(S)-malate
+
NADP+
=
oxaloacetate
+
NADPH
+
H+

Synonyms

(S)-malate dehydrogenase, dehydrogenase, malate (nicotinamide adenine dinucleotide phosphate), malate NADP dehydrogenase, malic dehydrogenase (nicotinamide adenine dinucleotide phosphate), MDH, NADP malate dehydrogenase, NADP-dependent malate dehydrogenase, NADP-linked malate dehydrogenase, NADP-malate dehydrogenase, NADP-malic enzyme, NADP-MDH, NADP-MDH1, NADP-MDH2, NADPH-MDH

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.82 malate dehydrogenase (NADP+)

Molecular Weight

Molecular Weight on EC 1.1.1.82 - malate dehydrogenase (NADP+)

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28400
-
2 * 28400, equilibrium sedimentation of the enzyme denatured by 6 M guanidinium chloride, pH 8, and 30 mM 2-mercaptoethanol
33000
Sorghum sp.
-
x * 33000, DELTAN, a truncation mutant corresponding to the deletion of the 5 end of the mdh cDNA open reading frame until the 73rd codon, SDS-PAGE
33489
4 * 34000, recombinant enzyme, SDS-PAGE, 4 * 33489, sequence calculation
34000
4 * 34000, recombinant enzyme, SDS-PAGE, 4 * 33489, sequence calculation
36000
-
2 * 36000, mutant DELTAN, lacking the N-terminal 45 residues, SDS-PAGE
38000
-
2 * 38000, SDS-PAGE
38500
-
1 * 38500, enzyme exists as monomer, dimer and tetramer, SDS-PAGE; 2 * 38500, enzyme exists as monomer, dimer and tetramer, SDS-PAGE; 4 * 38500, enzyme exists as monomer, dimer and tetramer, SDS-PAGE
38900
-
4 * 38900, native enzyme, SDS-PAGE
39000
-
2 * 39000, SDS-PAGE
40306
-
2 * 40306, enzyme from population Quebec, SDS-PAGE
41177
-
2 * 41177, enzyme from population Mississippi, SDS-PAGE
42000
-
2 * 42000, wild-type enzyme and mutant enzymes C23A, C28A, C23A/C28A, C23A/C28A/C206A, C23A/C28A/C364A, C23A/C28A/C376A, C23A/C28A/C206A/C364A, C23A/C28A/C206A/C376A and C23A/C28A/C364A/C376A, SDS-PAGE
42207
-
x * 42207, calculation from nucleotide sequence
50000 - 60000
-
unactivated enzyme, gel filtration
55000
-
equilibrium sedimentation
55680
-
equilibrium sedimentation
56000
-
gel filtration
57000
-
equilibrium sedimentation
61000
-
equilibrium sedimentation
72000
-
gel filtration
81000
-
gel filtration
83500
-
enzyme from population Quebec, non-denaturing PAGE
85450
-
isoenzyme 1 and 2 from Mississippi population, non-denaturing PAGE
86700
-
isoenzyme 3 from Mississippi population, non-denaturing PAGE
87000
-
dimer, gel filtration
88000
-
gradient pore-PAGE
91000
-
gel filtration
98000
-
gel filtration
110000 - 120000
-
unactivated enzyme, gel filtration
110000
recombinant enzyme, gel filtration
135000
-
gel filtration
143000 - 150000
-
activated enzyme, gel filtration
150000
-
gel filtration
163000
-
tetramer, gel filtration
additional information
-
-