Sequence of Q59127_PAENI
EC Number:1.5.99.4
EC Number
Recommended Name
Accession Code
Organism
No of amino acids
Molecular Weight [Da]
Source
Reaction
(S)-nicotine + acceptor + H2O = (S)-6-hydroxynicotine + reduced acceptor
Other sequences found for EC No. 1.5.99.4
General information:
Sequence
0 MKLPAIRYAS PASIEDACDL LSTDEDSKII AGGQSLLPVM AMRLAQPSVV IDLGNVPGLT
60 RLEEVGDYVR FGPMVTHATI VNSPAVAKHL PMMAAAGRHI AHPQIRNRGT LGGSLAHGDA
120 AGEWPLVLLA LNGMVEVQSV RGRRTIEADD LFVGPYMTSL AADEIITDVW IPSRPNGWGY
180 GEFARRSGDY GLANVAVVLS TADSRISDVR IAVGGAVGKI QRVPDAEDVL NGSELSPERA
240 EAASEAGAKS LSYISDIHGS AEYRHGLVQE LIRRTIVEAG ART
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Sequence Reference
Authors
Title
Journal
Volume
Pages
Year
PubMed ID
228497469
Brandsch R.,Hinkkanen A.E.,Mauch L.,Nagursky H.,Decker K.
6-hydroxy-D-nicotine oxidase of Arthrobacter oxidans. Gene structure of the flavoenzyme and its relationship to 6-hydroxy-L-nicotine oxidase.
Eur. J. Biochem.
167
315-320
1987
228497470
Grether-Beck S.,Igloi G.L.,Pust S.,Schiltz E.,Decker K.,Brandsch R.
Structural analysis and molybdenum-dependent expression of the pAO1-encoded nicotine dehydrogenase genes of Arthrobacter nicotinovorans.
Mol. Microbiol.
13
929-936
1994
228497471
Menendez C.,Igloi G.,Henninger H.,Brandsch R.
A pAO1-encoded molybdopterin cofactor gene (moaA) of Arthrobacter nicotinovorans: characterization and site-directed mutagenesis of the encoded protein.
Arch. Microbiol.
164
142-151
1995
228497473
Menendez C.,Otto A.,Igloi G.,Nick P.,Brandsch R.,Schubach B.,Bottcher B.,Brandsch R.
Molybdate-uptake genes and molybdopterin-biosynthesis genes on a bacterial plasmid: characterization of MoeA as a filament-forming protein with adenosinetriphosphatase activity.
Eur. J. Biochem.
250
524-531
1997
228497474
Menendez C.,Igloi G.L.,Brandsch R.
IS1473, a putative insertion sequence identified in the plasmid pAO1 from Arthrobacter nicotinovorans: isolation, characterization, and distribution among Arthrobacter species.
Plasmid
37
35-41
1997
228497475
Baitsch D.,Sandu C.,Brandsch R.,Igloi G.L.
Gene cluster on pAO1 of Arthrobacter nicotinovorans involved in degradation of the plant alkaloid nicotine: cloning, purification, and characterization of 2,6-dihydroxypyridine 3-hydroxylase.
J. Bacteriol.
183
5262-5267
2001
228497476
Igloi G.L.,Brandsch R.
Sequence of the 165-kilobase catabolic plasmid pAO1 from Arthrobacter nicotinovorans and identification of a pAO1-dependent nicotine uptake system.
J. Bacteriol.
185
1976-1986
2003
228497477
Sandu C.,Chiribau C.B.,Brandsch R.
Characterization of HdnoR, the transcriptional repressor of the 6-hydroxy-D-nicotine oxidase gene of Arthrobacter nicotinovorans pAO1, and its DNA-binding activity in response to L- and D-nicotine Derivatives.
J. Biol. Chem.
278
51307-51315
2003
228497478
Chiribau CB.,Sandu C.,Fraaije M.,Schiltz E.,Brandsch R.
A novel gamma-N-methylaminobutyrate demethylating oxidase involved in catabolism of the tobacco alkaloid nicotine by Arthrobacter nicotinovorans pAO1.
Eur. J. Biochem.
271
4677-4684
2004
228497479
Chiribau CB.,Sandu C.,Igloi G.L.,Brandsch R.
Characterization of PmfR, the transcriptional activator of the pAO1-borne purU-mabO-folD operon of Arthrobacter nicotinovorans.
J. Bacteriol.
187
3062-3070
2005
228497480
Sachelaru P.,Schiltz E.,Igloi G.L.,Brandsch R.
An alpha/beta-fold C--C bond hydrolase is involved in a central step of nicotine catabolism by Arthrobacter nicotinovorans.
J. Bacteriol.
187
8516-8519
2005
228497481
Sachelaru P.,Schiltz E.,Brandsch R.
A functional mobA gene for molybdopterin cytosine dinucleotide cofactor biosynthesis is required for activity and holoenzyme assembly of the heterotrimeric nicotine dehydrogenases of Arthrobacter nicotinovorans.
Appl. Environ. Microbiol.
72
5126-5131
2006
228497482
Brandsch R.
Microbiology and biochemistry of nicotine degradation.
Appl. Microbiol. Biotechnol.
69
493-498
2006
228497483
Chiribau CB.,Mihasan M.,Ganas P.,Igloi G.L.,Artenie V.,Brandsch R.
Final steps in the catabolism of nicotine.
FEBS J.
273
1528-1536
2006
228497484
Mihasan M.,Chiribau CB.,Friedrich T.,Artenie V.,Brandsch R.
An NAD(P)H-nicotine blue oxidoreductase is part of the nicotine regulon and may protect Arthrobacter nicotinovorans from oxidative stress during nicotine catabolism.
Appl. Environ. Microbiol.
73
2479-2485
2007
228497485
Ganas P.,Mihasan M.,Igloi GL.,Brandsch R.
A two-component small multidrug resistance pump functions as a metabolic valve during nicotine catabolism by Arthrobacter nicotinovorans.
Microbiology
153
1546-1555
2007
228497486
Cobzaru C.,Ganas P.,Mihasan M.,Schleberger P.,Brandsch R.
Homologous gene clusters of nicotine catabolism, including a new omega-amidase for alpha-ketoglutaramate, in species of three genera of Gram-positive bacteria.
Res. Microbiol.
162
285-291
2011
228497487
Mihasan M.,Brandsch R.
pAO1 of Arthrobacter nicotinovorans and the spread of catabolic traits by horizontal gene transfer in gram-positive soil bacteria.
J. Mol. Evol.
77
22-30
2013
228497488
Mihasan M.,Stefan M.,Hritcu L.,Artenie V.,Brandsch R.
Evidence of a plasmid-encoded oxidative xylose-catabolic pathway in Arthrobacter nicotinovorans pAO1.
Res. Microbiol.
164
22-30
2013
