Sequence of COLH_HATHI
EC Number:3.4.24.3
EC Number
Recommended Name
Accession Code
Organism
No of amino acids
Molecular Weight [Da]
Source
Reaction
Digestion of native collagen in the triple helical region at -/-Gly bonds. With synthetic peptides, a preference is shown for Gly at P3 and P1', Pro and Ala at P2 and P2', and hydroxyproline, Ala or Arg at P3'
Other sequences found for EC No. 3.4.24.3
General information:
Sequence
0 MKRKCLSKRL MLAITMATIF TVNSTLPIYA AVDKNNATAA VQNESKRYTV SYLKTLNYYD
60 LVDLLVKTEI ENLPDLFQYS SDAKEFYGNK TRMSFIMDEI GRRAPQYTEI DHKGIPTLVE
120 VVRAGFYLGF HNKELNEINK RSFKERVIPS ILAIQKNPNF KLGTEVQDKI VSATGLLAGN
180 ETAPPEVVNN FTPILQDCIK NIDRYALDDL KSKALFNVLA APTYDITEYL RATKEKPENT
240 PWYGKIDGFI NELKKLALYG KINDNNSWII DNGIYHIAPL GKLHSNNKIG IETLTEVMKV
300 YPYLSMQHLQ SADQIKRHYD SKDAEGNKIP LDKFKKEGKE KYCPKTYTFD DGKVIIKAGA
360 RVEEEKVKRL YWASKEVNSQ FFRVYGIDKP LEEGNPDDIL TMVIYNSPEE YKLNSVLYGY
420 DTNNGGMYIE PEGTFFTYER EAQESTYTLE ELFRHEYTHY LQGRYAVPGQ WGRTKLYDND
480 RLTWYEEGGA ELFAGSTRTS GILPRKSIVS NIHNTTRNNR YKLSDTVHSK YGASFEFYNY
540 ACMFMDYMYN KDMGILNKLN DLAKNNDVDG YDNYIRDLSS NYALNDKYQD HMQERIDNYE
600 NLTVPFVADD YLVRHAYKNP NEIYSEISEV AKLKDAKSEV KKSQYFSTFT LRGSYTGGAS
660 KGKLEDQKAM NKFIDDSLKK LDTYSWSGYK TLTAYFTNYK VDSSNRVTYD VVFHGYLPNE
720 GDSKNSLPYG KINGTYKGTE KEKIKFSSEG SFDPDGKIVS YEWDFGDGNK SNEENPEHSY
780 DKVGTYTVKL KVTDDKGESS VSTTTAEIKD LSENKLPVIY MHVPKSGALN QKVVFYGKGT
840 YDPDGSIAGY QWDFGDGSDF SSEQNPSHVY TKKGEYTVTL RVMDSSGQMS EKTMKIKITD
900 PVYPIGTEKE PNNSKETASG PIVPGIPVSG TIENTSDQDY FYFDVITPGE VKIDINKLGY
960 GGATWVVYDE NNNAVSYATD DGQNLSGKFK ADKPGRYYIH LYMFNGSYMP YRINIEGSVG
1020 R
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Sequence Reference
Authors
Title
Journal
Volume
Pages
Year
PubMed ID
912315
Yoshihara K.,Matsushita O.,Minami J.,Okabe A.
Cloning and nucleotide sequence analysis of the colH gene from Clostridium histolyticum encoding a collagenase and a gelatinase.
J. Bacteriol.
176
6489-6496
1994
912316
Matsushita O.,Jung C.-M.,Katayama S.,Minami J.,Takahashi Y.,Okabe A.
Gene duplication and multiplicity of collagenases in Clostridium histolyticum.
J. Bacteriol.
181
923-933
1999
912317
Bond M.D.,Van Wart H.E.
Characterization of the individual collagenases from Clostridium histolyticum.
Biochemistry
23
3085-3091
1984
912318
Mookhtiar K.A.,Steinbrink D.R.,Van Wart H.E.
Mode of hydrolysis of collagen-like peptides by class I and class II Clostridium histolyticum collagenases: evidence for both endopeptidase and tripeptidylcarboxypeptidase activities.
Biochemistry
24
6527-6533
1985
912319
Gelbard M.K.,James K.,Riach P.,Dorey F.
Collagenase versus placebo in the treatment of Peyronie's disease: a double-blind study.
J. Urol.
149
56-58
1993
912320
Matsushita O.,Jung C.M.,Minami J.,Katayama S.,Nishi N.,Okabe A.
A study of the collagen-binding domain of a 116-kDa Clostridium histolyticum collagenase.
J. Biol. Chem.
273
3643-3648
1998
912321
Fecteau K.A.,Haffner J.C.,Eiler H.
The potential of collagenase as a new therapy for separation of human retained placenta: hydrolytic potency on human, equine and bovine placentae.
Placenta
19
379-383
1998
912322
Nishi N.,Matsushita O.,Yuube K.,Miyanaka H.,Okabe A.,Wada F.
Collagen-binding growth factors: production and characterization of functional fusion proteins having a collagen-binding domain.
Proc. Natl. Acad. Sci. U.S.A.
95
7018-7023
1998
912323
Jung C.M.,Matsushita O.,Katayama S.,Minami J.,Sakurai J.,Okabe A.
Identification of metal ligands in the Clostridium histolyticum ColH collagenase.
J. Bacteriol.
181
2816-2822
1999
912324
Badalamente M.A.,Hurst L.C.
Enzyme injection as nonsurgical treatment of Dupuytren's disease.
J. Hand Surg. Am.
25
629-636
2000
912325
McCarthy R.C.,Spurlin B.,Wright M.J.,Breite A.G.,Sturdevant L.K.,Dwulet C.S.,Dwulet F.E.
Development and characterization of a collagen degradation assay to assess purified collagenase used in islet isolation.
Transplant. Proc.
40
339-342
2008
912326
Eckhard U.,Schoenauer E.,Ducka P.,Briza P.,Nuess D.,Brandstetter H.
Biochemical characterization of the catalytic domains of three different Clostridial collagenases.
Biol. Chem.
390
11-18
2009
912327
Shi L.,Carson D.
Collagenase Santyl ointment: a selective agent for wound debridement.
J. Wound Ostomy Continence Nurs.
36
0-0
2009
912328
Hurst L.C.,Badalamente M.A.,Hentz V.R.,Hotchkiss R.N.,Kaplan F.T.,Meals R.A.,Smith T.M.,Rodzvilla J.
Injectable collagenase clostridium histolyticum for Dupuytren's contracture.
N. Engl. J. Med.
361
968-979
2009
912329
Breite A.G.,McCarthy R.C.,Dwulet F.E.
Characterization and functional assessment of Clostridium histolyticum class I (C1) collagenases and the synergistic degradation of native collagen in enzyme mixtures containing class II (C2) collagenase.
Transplant. Proc.
43
3171-3175
2011
912330
Ohbayashi N.,Matsumoto T.,Shima H.,Goto M.,Watanabe K.,Yamano A.,Katoh Y.,Igarashi K.,Yamagata Y.,Murayama K.
Solution structure of clostridial collagenase H and its calcium-dependent global conformation change.
Biophys. J.
104
1538-1545
2013
912331
Eckhard U.,Huesgen P.F.,Brandstetter H.,Overall C.M.
Proteomic protease specificity profiling of clostridial collagenases reveals their intrinsic nature as dedicated degraders of collagen.
J. Proteomics
100
102-114
2014
912332
Lipshultz L.I.,Goldstein I.,Seftel A.D.,Kaufman G.J.,Smith T.M.,Tursi J.P.,Burnett A.L.
Clinical efficacy of collagenase Clostridium histolyticum in the treatment of Peyronie's disease by subgroup: results from two large, double-blind, randomized, placebo-controlled, phase III studies.
BJU Int.
116
650-656
2015
912333
Bauer R.,Wilson J.J.,Philominathan S.T.,Davis D.,Matsushita O.,Sakon J.
Structural comparison of ColH and ColG collagen-binding domains from Clostridium histolyticum.
J. Bacteriol.
195
318-327
2013
912334
Eckhard U.,Schonauer E.,Brandstetter H.
Structural basis for activity regulation and substrate preference of clostridial collagenases G, H, and T.
J. Biol. Chem.
288
20184-20194
2013
912335
Bauer R.,Janowska K.,Taylor K.,Jordan B.,Gann S.,Janowski T.,Latimer E.C.,Matsushita O.,Sakon J.
Structures of three polycystic kidney disease-like domains from Clostridium histolyticum collagenases ColG and ColH.
Acta Crystallogr. D
71
565-577
2015
912336
Schoenauer E.,Kany A.M.,Haupenthal J.,Huesecken K.,Hoppe I.J.,Voos K.,Yahiaoui S.,Elsaesser B.,Ducho C.,Brandstetter H.,Hartmann R.W.
Discovery of a potent inhibitor class with high selectivity toward clostridial collagenases.
J. Am. Chem. Soc.
139
12696-12703
2017
