EC Number   |
Protein Variants |
Reference |
|---|
    6.3.2.49 | more |
generation of a deletion mutant comprising residues Lys4-Tyr468 |
-, 726615 |
| 6.3.2.49 | more |
several bacilysin-deficient strains, e.g. strain ng79, construction of mutant strains GSB315, GSB319, GSB320, GSB321, and GSB322 |
-, 733219 |
| 6.3.2.49 | S1845A |
no significant difference in kcat value between the wild type and the mutant enzyme. 2fold increase in Km-value for L-alanine |
-, 730896 |
| 6.3.2.49 | Y332A |
site-directed mutagenesis, mutation of Trp332 to Ala causes the enzyme to hydrolyze ATP, even in the absence of L-Ala, and the structure of this mutant protein show a cavity in the N-terminal substrate-binding pocket |
733347 |
| 6.3.2.49 | Y75F |
no significant difference in kcat value between the wild type and the mutant enzyme, 2fold increase in KM-value for L-phenylalanine |
-, 730896 |
