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Information on EC 6.3.2.49 - L-alanine-L-anticapsin ligase
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6.3.2.49 L-alanine-L-anticapsin ligaseIUBMB Comments
The enzyme, characterized from the bacterium Bacillus subtilis, is involved in the biosynthesis of the nonribosomally synthesized dipeptide antibiotic bacilysin, composed of L-alanine and L-anticapsin. The enzyme requires Mg2+ or Mn2+ for activity, and has a broad substrate specificity in vitro .
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Word Map
- 6.3.2.49
- dipeptide
-
unprotected
- l-alanyl-l-glutamine
- oligopeptide
-
atp-grasp
-
rhizocticins
- ligases
- licheniformis
- bacilysin
- synthesis
-
branched-chain
- syringae
- alpha-peptide
-
taste
-
d-amino
-
homo-oligomers
- l-glutamine
- adolescentis
- met-gly
- l-asparagine
- ala-gln
- pharmacology
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
l-amino acid ligase, bl00235, l-amino acid alpha-ligase, rsp1486a, l-amino-acid ligase, lal bl00235, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
alanine-anticapsin ligase
BacD
BL00235
BSU37710
EC 6.3.2.28
-
-
formerly
-
L-Ala-L-aniticapsin ligase
L-amino acid alpha-ligase
L-amino acid ligase
L-amino-acid ligase
LAL
LAL BL00235
RSp1486a
TabS
YwfE
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ATP + L-alanine + L-anticapsin = ADP + phosphate + bacilysin
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
BRENDA
-
-
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SYSTEMATIC NAME
IUBMB Comments
L-alanine:L-anticapsin ligase (ADP-forming)
The enzyme, characterized from the bacterium Bacillus subtilis, is involved in the biosynthesis of the nonribosomally synthesized dipeptide antibiotic bacilysin, composed of L-alanine and L-anticapsin. The enzyme requires Mg2+ or Mn2+ for activity, and has a broad substrate specificity in vitro [1].
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(2S)-3-[[(1R)-1-aminoethyl(hydroxy)]phosphoryl]-2-benzylpropionic acid + ATP
(2S)-3-[(3)-[(1R)-1-aminoethyl(phosphonooxy)]phosphoryl]-2-benzylpropanoic acid + ADP
ATP + 2 L-cysteine
ADP + phosphate + L-cysteinyl-L-cysteine
-
assay at pH 8.0, 37°C
-
-
?
ATP + alanine + alanine
ADP + phosphate + L-alanyl-L-alanine
-
assay at pH 8.0, 37°C
-
-
?
ATP + arginine + histidine
ADP + phosphate + L-arginyl-L-histidine
-
assay at pH 8.0, 37°C
-
-
?
ATP + arginine + methionine
ADP + phosphate + L-arginyl-L-methionine
-
assay at pH 8.0, 37°C
-
-
?
ATP + arginine + tyrosine
ADP + phosphate + L-arginyl-L-tyrosine
-
assay at pH 8.0, 37°C
-
-
?
ATP + beta-alanine + beta-alanine
ADP + phosphate + beta-alanyl-beta-alanine
-
-
-
?
ATP + cis-4-hydroxy-L-proline + L-threonine
ADP + phosphate + cis-4-hydroxy-L-prolyl-L-threonine
-
-
-
?
ATP + cysteine + alanine
ADP + phosphate + L-cysteinyl-L-alanine
-
assay at pH 8.0, 37°C
-
-
?
ATP + cysteine + serine
ADP + phosphate + L-cysteinyl-L-serine
-
assay at pH 8.0, 37°C
-
-
?
ATP + cysteine + threonine
ADP + phosphate + L-cysteinyl-L-threonine
-
assay at pH 8.0, 37°C
-
-
?
ATP + glutamic acid + leucine
ADP + phosphate + ?
-
assay at pH 8.0, 37°C
-
-
?
ATP + glutamine + glutamic acid
ADP + phosphate + ?
assay at pH 8.0, 37°C
-
-
?
ATP + glutamine + phenylalanine
ADP + phosphate + L-glutaminyl-L-phenylalanine
assay at pH 8.0, 37°C
-
-
?
ATP + glycine + L-proline
ADP + phosphate + glycyl-L-proline
assay at pH 8.0, 37°C
-
-
?
ATP + histidine + asparagine
ADP + phosphate + L-histidyl-L-asparagine
-
assay at pH 8.0, 37°C
-
-
?
ATP + histidine + glutamine
ADP + phosphate + L-histidyl-L-glutamine
-
assay at pH 8.0, 37°C
-
-
?
ATP + histidine + histidine
ADP + phosphate + L-histidyl-L-histidine
-
assay at pH 8.0, 37°C
-
-
?
ATP + histidine + methionine
ADP + phosphate + L-histidyl-L-methionine
-
assay at pH 8.0, 37°C
-
-
?
ATP + histidine + tryptophan
ADP + phosphate + L-histidyl-L-tryptophan
-
assay at pH 8.0, 37°C
-
-
?
ATP + histidine + valine
ADP + phosphate + L-histidyl-L-valine
-
assay at pH 8.0, 37°C
-
-
?
ATP + isoleucine + alanine
ADP + phosphate + L-isoleucyl-L-alanine
-
assay at pH 8.0, 37°C
-
-
?
ATP + isoleucine + cysteine
ADP + phosphate + L-isoleucyl-L-cysteine
-
assay at pH 8.0, 37°C
-
-
?
ATP + isoleucine + serine
ADP + phosphate + L-isoleucyl-L-serine
-
assay at pH 8.0, 37°C
-
-
?
ATP + L-alanine + 3-[(1S)-4-oxocyclohex-2-en-1-yl]-L-alanine
ADP + phosphate + L-alanyl-[3-[(1S)-4-oxocyclohex-2-en-1-yl]-L-alanine]
ATP + L-arginine + beta-alanine
ADP + phosphate + beta-alanyl-L-arginine
-
-
-
?
ATP + L-arginine + L-phenylalanine
ADP + phosphate + L-arginyl-L-phenylalanine
-
-
-
?
ATP + L-asparagine + L-glutamine
ADP + phosphate + L-asparaginyl-L-glutamine
-
-
-
?
ATP + L-asparagine + L-isoleucine
ADP + phosphate + L-asparaginyl-L-isoleucine
-
-
-
?
ATP + L-asparagine + L-serine
ADP + phosphate + L-asparaginyl-L-serine
-
-
-
?
ATP + L-asparagine + L-threonine
ADP + phosphate + L-asparaginyl-L-threonine
-
-
-
?
ATP + L-asparagine + L-tryptophan
ADP + phosphate + L-asparaginyl-L-tryptophan
-
assay at pH 8.0, 37°C
-
-
?
ATP + L-asparagine + L-valine
ADP + phosphate + L-asparaginyl-L-valine
-
-
-
?
ATP + L-aspartate + L-alanine
ADP + phosphate + L-aspartyl-L-alanine
-
-
-
?
ATP + L-aspartate + L-isoleucine
ADP + phosphate + L-aspartyl-L-isoleucine
-
-
-
?
ATP + L-aspartate + L-leucine
ADP + phosphate + L-aspartyl-L-leucine
-
-
-
?
ATP + L-aspartate + L-methionine
ADP + phosphate + L-aspartyl-L-methionine
-
-
-
?
ATP + L-azetidine-2-carboxylate + L-threonine
ADP + phosphate + L-azetidine-2-carboxyl-L-threonine
-
-
-
?
ATP + L-cysteine + glycine
ADP + phosphate + L-cysteinyl-glycine
-
assay at pH 8.0, 37°C
-
-
?
ATP + L-glutamate + beta-alanine
ADP + phosphate + beta-alanyl-L-glutamine
-
-
-
?
ATP + L-glutamate + L-methionine
ADP + phosphate + L-glutamyl-L-methionine
-
-
-
?
ATP + L-glutamine + L-arginine
ADP + phosphate + L-glutaminyl-L-arginine
-
-
-
?
ATP + L-glutamine + L-histidine
ADP + phosphate + L-glutaminyl-L-histidine
-
-
-
?
ATP + L-glutamine + L-isoleucine
ADP + phosphate + L-glutaminyl-L-isoleucine
-
-
-
?
ATP + L-glutamine + L-serine
ADP + phosphate + L-glutaminyl-L-serine
-
-
-
?
ATP + L-glutamine + L-threonine
ADP + phosphate + L-glutaminyl-L-threonine
-
-
-
?
ATP + L-glutamine + L-valine
ADP + phosphate + L-glutaminyl-L-valine
-
-
-
?
ATP + L-histidine + glycine
ADP + phosphate + L-histidyl-glycine
-
assay at pH 8.0, 37°C
-
-
?
ATP + L-isoleucine + beta-alanine
ADP + phosphate + beta-alanyl-L-isoleucine
-
-
-
?
ATP + L-leucine + beta-alanine
ADP + phosphate + beta-alanyl-L-leucine
-
-
-
?
ATP + L-leucine + L-isoleucine
ADP + phosphate + L-leucyl-L-isoleucine
-
-
-
?
ATP + L-leucine + L-serine
ADP + phosphate + L-leucyl-L-serine
-
-
-
?
ATP + L-methionine + beta-alanine
ADP + phosphate + beta-alanyl-L-methionine
-
-
-
?
ATP + L-methionine + L-alanine
ADP + phosphate + L-methionyl-L-alanine
-
best substrate combination
-
-
?
ATP + L-methionine + L-cysteine
ADP + phosphate + L-methionyl-L-cysteine
-
-
-
-
?
ATP + L-methionine + L-leucine
ADP + phosphate + L-methionyl-L-leucine
-
-
-
-
?
ATP + L-methionine + L-methionine
ADP + phosphate + L-methionyl-L-methionine
-
-
-
-
?
ATP + L-phenylalanine + glycine
ADP + phosphate + L-phenylalanylglycine
-
-
-
?
ATP + L-phenylalanine + L-alanine
ADP + phosphate + L-phenylalanyl-L-alanine
-
-
-
?
ATP + L-phenylalanine + L-arginine
ADP + phosphate + L-phenylalanyl-L-arginine
-
-
-
?
ATP + L-phenylalanine + L-asparagine
ADP + phosphate + L-phenylalanyl-L-asparagine
-
-
-
?
ATP + L-phenylalanine + L-glutamine
ADP + phosphate + L-phenylalanyl-L-glutamine
-
-
-
?
ATP + L-phenylalanine + L-histidine
ADP + phosphate + L-phenylalanyl-L-histidine
-
-
-
?
ATP + L-phenylalanine + L-isoleucine
ADP + phosphate + L-phenylalanyl-L-isoleucine
-
-
-
?
ATP + L-phenylalanine + L-leucine
ADP + phosphate + L-phenylalanyl-L-leucine
-
-
-
?
ATP + L-phenylalanine + L-lysine
ADP + phosphate + L-phenylalanyl-L-lysine
-
-
-
?
ATP + L-phenylalanine + L-methionine
ADP + phosphate + L-phenylalanyl-L-methionine
-
-
-
?
ATP + L-phenylalanine + L-phenylalanine
ADP + phosphate + L-phenylalanyl-L-phenylalanine
-
-
-
?
ATP + L-phenylalanine + L-proline
ADP + phosphate + L-phenylalanyl-L-proline
-
-
-
?
ATP + L-phenylalanine + L-serine
ADP + phosphate + L-phenylalanyl-L-serine
-
-
-
?
ATP + L-phenylalanine + L-threonine
ADP + phosphate + L-phenylalanyl-L-threonine
-
-
-
?
ATP + L-phenylalanine + L-tryptophan
ADP + phosphate + L-phenylalanyl-L-tryptophan
-
-
-
?
ATP + L-phenylalanine + L-valine
ADP + phosphate + L-phenylalanyl-L-valine
-
-
-
?
ATP + L-pipecolic acid + L-threonine
ADP + phosphate + L-pipecoloyl-L-threonine
-
-
-
?
ATP + L-proline + beta-alanine
ADP + phosphate + beta-alanyl-L-proline
-
-
-
?
ATP + L-proline + glycine
ADP + phosphate + L-prolylglycine
-
-
-
?
ATP + L-proline + L-alanine
ADP + phosphate + L-prolyl-L-alanine
-
-
-
?
ATP + L-proline + L-asparagine
ADP + phosphate + L-prolyl-L-asparagine
-
-
-
?
ATP + L-proline + L-aspartate
ADP + phosphate + L-prolyl-L-aspartate
-
-
-
?
ATP + L-proline + L-glutamate
ADP + phosphate + L-prolyl-L-glutamate
-
-
-
?
ATP + L-proline + L-glutamine
ADP + phosphate + L-prolyl-L-glutamine
-
-
-
?
ATP + L-proline + L-isoleucine
ADP + phosphate + L-prolyl-L-isoleucine
-
-
-
?
ATP + L-proline + L-leucine
ADP + phosphate + L-prolyl-L-leucine
-
-
-
?
ATP + L-proline + L-methionine
ADP + phosphate + L-prolyl-L-methionine
-
-
-
?
ATP + L-proline + L-serine
ADP + phosphate + L-prolyl-L-serine
-
-
-
?
ATP + L-proline + L-threonine
ADP + phosphate + L-prolyl-L-threonine
-
-
-
?
ATP + L-proline + L-valine
ADP + phosphate + L-prolyl-L-valine
-
-
-
?
ATP + L-serine + L-methionine
ADP + phosphate + L-seryl-L-methionine
-
-
-
-
?
ATP + L-threonine + beta-alanine
ADP + phosphate + beta-alanyl-L-threonine
-
-
-
?
ATP + L-threonine + L-methionine
ADP + phosphate + L-threonyl-L-methionine
-
-
-
-
?
ATP + L-tryptophan + beta-alanine
ADP + phosphate + beta-alanyl-L-tryptophan
-
-
-
?
ATP + L-tryptophan + glycine
ADP + phosphate + L-tryptophylglycine
-
-
-
?
ATP + L-tryptophan + L-alanine
ADP + phosphate + L-tryptophyl-L-alanine
-
-
-
?
ATP + L-tryptophan + L-arginine
ADP + phosphate + L-tryptophyl-L-arginine
-
-
-
?
ATP + L-tryptophan + L-asparagine
ADP + phosphate + L-tryptophyl-L-asparagine
-
-
-
?
ATP + L-tryptophan + L-aspartate
ADP + phosphate + L-tryptophyl-L-aspartate
-
-
-
?
ATP + L-tryptophan + L-glutamate
ADP + phosphate + L-tryptophyl-L-glutamate
-
-
-
?
ATP + L-tryptophan + L-glutamine
ADP + phosphate + L-tryptophyl-L-glutamine
-
-
-
?
ATP + L-tryptophan + L-histidine
ADP + phosphate + L-tryptophyl-L-histidine
-
-
-
?
ATP + L-tryptophan + L-isoleucine
ADP + phosphate + L-tryptophyl-L-isoleucine
-
-
-
?
ATP + L-tryptophan + L-leucine
ADP + phosphate + L-tryptophyl-L-leucine
-
-
-
?
ATP + L-tryptophan + L-lysine
ADP + phosphate + L-tryptophyl-L-lysine
-
-
-
?
ATP + L-tryptophan + L-methionine
ADP + phosphate + L-tryptophyl-L-methionine
-
-
-
?
ATP + L-tryptophan + L-proline
ADP + phosphate + L-tryptophyl-L-proline
-
-
-
?
ATP + L-tryptophan + L-serine
ADP + phosphate + L-tryptophyl-L-serine
-
-
-
?
ATP + L-tryptophan + L-threonine
ADP + phosphate + L-tryptophyl-L-threonine
-
-
-
?
ATP + L-tryptophan + L-tryptophan
ADP + phosphate + L-tryptophyl-L-tryptophan
-
-
-
?
ATP + L-tryptophan + L-valine
ADP + phosphate + L-tryptophyl-L-valine
-
-
-
?
ATP + L-tyrosine + glycine
ADP + phosphate + L-tyrosinyl-glycine
-
assay at pH 8.0, 37°C
-
-
?
ATP + L-tyrosine + L-arginine
ADP + phosphate + L-tyrosyl-L-arginine
-
-
-
?
ATP + L-tyrosine + L-glutamine
ADP + phosphate + L-tyrosyl-L-glutamine
-
-
-
?
ATP + L-tyrosine + L-histidine
ADP + phosphate + L-tyrosyl-L-histidine
-
-
-
?
ATP + L-tyrosine + L-isoleucine
ADP + phosphate + L-tyrosyl-L-isoleucine
-
-
-
?
ATP + L-tyrosine + L-methionine
ADP + phosphate + L-tyrosyl-L-methionine
-
-
-
?
ATP + L-tyrosine + L-phenylalanine
ADP + phosphate + L-tyrosyl-L-phenylalanine
-
-
-
?
ATP + L-tyrosine + L-proline
ADP + phosphate + L-tyrosyl-L-proline
-
-
-
?
ATP + L-tyrosine + L-threonine
ADP + phosphate + L-tyrosyl-L-threonine
-
-
-
?
ATP + L-tyrosine + L-tryptophan
ADP + phosphate + L-tyrosyl-L-tryptophan
-
-
-
?
ATP + L-tyrosine + L-valine
ADP + phosphate + L-tyrosyl-L-valine
-
-
-
?
ATP + L-valine + beta-alanine
ADP + phosphate + beta-alanyl-L-valine
-
-
-
?
ATP + leucine + alanine
ADP + phosphate + L-leucyl-L-alanine
-
assay at pH 8.0, 37°C
-
-
?
ATP + leucine + serine
ADP + phosphate + L-leucyl-L-serine
-
assay at pH 8.0, 37°C
-
-
?
ATP + leucine + threonine
ADP + phosphate + L-leucyl-L-threonine
-
assay at pH 8.0, 37°C
-
-
?
ATP + lysine + histidine
ADP + phosphate + L-lysyl-L-histidine
-
assay at pH 8.0, 37°C
-
-
?
ATP + lysine + phenylalanine
ADP + phosphate + L-lysyl-L-phenylalanine
-
assay at pH 8.0, 37°C
-
-
?
ATP + methionine + alanine
ADP + phosphate + L-methionyl-L-alanine
-
assay at pH 8.0, 37°C
-
-
?
ATP + methionine + isoleucine
ADP + phosphate + L-methionyl-L-isoleucine
-
assay at pH 8.0, 37°C
-
-
?
ATP + methionine + leucine
ADP + phosphate + L-methionyl-L-leucine
-
assay at pH 8.0, 37°C
-
-
?
ATP + methionine + methionine
ADP + phosphate + L-methionyl-L-methionine
-
assay at pH 8.0, 37°C
-
-
?
ATP + methionine + serine
ADP + phosphate + L-methionyl-L-serine
-
assay at pH 8.0, 37°C
-
-
?
ATP + methionine + threonine
ADP + phosphate + L-methionyl-L-threonine
-
assay at pH 8.0, 37°C
-
-
?
ATP + methionine + valine
ADP + phosphate + L-methionyl-L-valine
-
assay at pH 8.0, 37°C
-
-
?
ATP + phenylalanine + isoleucine
ADP + phosphate + L-phenylalanyl-L-isoleucine
-
assay at pH 8.0, 37°C
-
-
?
ATP + phenylalanine + threonine
ADP + phosphate + L-phenylalanyl-L-threonine
-
assay at pH 8.0, 37°C
-
-
?
ATP + phenylalanine + valine
ADP + phosphate + L-phenylalanyl-L-valine
-
assay at pH 8.0, 37°C
-
-
?
ATP + serine + alanine
ADP + phosphate + L-serinyl-L-alanine
-
assay at pH 8.0, 37°C
-
-
?
ATP + serine + serine
ADP + phosphate + L-serinyl-L-serine
-
assay at pH 8.0, 37°C
-
-
?
ATP + trans-4-hydroxy-L-proline + L-threonine
ADP + phosphate + trans-4-hydroxy-L-prolyl-L-threonine
-
-
-
?
ATP + tryptophan + isoleucine
ADP + phosphate + L-tryptophanyl-L-isoleucine
-
assay at pH 8.0, 37°C
-
-
?
ATP + tryptophan + leucine
ADP + phosphate + L-tryptophanyl-L-leucine
-
assay at pH 8.0, 37°C
-
-
?
ATP + tryptophan + methionine
ADP + phosphate + L-tryptophanyl-L-methionine
-
assay at pH 8.0, 37°C
-
-
?
ATP + tryptophan + phenylalanine
ADP + phosphate + L-tryptophanyl-L-phenylalanine
-
assay at pH 8.0, 37°C
-
-
?
ATP + tryptophan + threonine
ADP + phosphate + L-tryptophanyl-L-threonine
-
assay at pH 8.0, 37°C
-
-
?
ATP + tryptophan + tryptophan
ADP + phosphate + L-tryptophanyl-L-tryptophan
-
assay at pH 8.0, 37°C
-
-
?
ATP + tryptophan + valine
ADP + phosphate + L-tryptophanyl-L-valine
-
assay at pH 8.0, 37°C
-
-
?
ATP + tyrosine + cysteine
ADP + phosphate + L-tyrosinyl-L-cysteine
-
assay at pH 8.0, 37°C
-
-
?
ATP + tyrosine + methionine
ADP + phosphate + L-tyrosinyl-L-methionine
-
assay at pH 8.0, 37°C
-
-
?
ATP + tyrosine + phenylalanine
ADP + phosphate + L-tyrosinyl-L-phenylalanine
-
assay at pH 8.0, 37°C
-
-
?
ATP + tyrosine + serine
ADP + phosphate + L-tyrosinyl-L-serine
-
assay at pH 8.0, 37°C
-
-
?
ATP + valine + alanine
ADP + phosphate + L-valinyl-L-alanine
-
assay at pH 8.0, 37°C
-
-
?
ATP + valine + serine
ADP + phosphate + L-valinyl-L-serine
-
assay at pH 8.0, 37°C
-
-
?
(2S)-3-[[(1R)-1-aminoethyl(hydroxy)]phosphoryl]-2-benzylpropionic acid + ATP
(2S)-3-[(3)-[(1R)-1-aminoethyl(phosphonooxy)]phosphoryl]-2-benzylpropanoic acid + ADP
the phosphinate L-alanyl-L-phenylalanine analog binds the active site of the enzyme, and accepts a phosphate group from ATP to form the phosphorylated phosphinate L-alanyl-L-phenylalanine analog
-
-
?
(2S)-3-[[(1R)-1-aminoethyl(hydroxy)]phosphoryl]-2-benzylpropionic acid + ATP
(2S)-3-[(3)-[(1R)-1-aminoethyl(phosphonooxy)]phosphoryl]-2-benzylpropanoic acid + ADP
the phosphinate L-alanyl-L-phenylalanine analog binds the active site of the enzyme, and accepts a phosphate group from ATP to form the phosphorylated phosphinate L-alanyl-L-phenylalanine analog
-
-
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ATP + an L-amino acid + an L-amino acid
ADP + phosphate + L-aminoacyl-L-amino acid
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ATP + an L-amino acid + an L-amino acid
ADP + phosphate + L-aminoacyl-L-amino acid
the enzyme catalyzes the formation of an alpha-peptide bond between two L-amino acids in an ATP-dependent manner
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ATP + an L-amino acid + an L-amino acid
ADP + phosphate + L-aminoacyl-L-amino acid
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?
ATP + an L-amino acid + an L-amino acid
ADP + phosphate + L-aminoacyl-L-amino acid
the enzyme catalyzes the formation of an alpha-peptide bond between two L-amino acids in an ATP-dependent manner
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ATP + an L-amino acid + an L-amino acid
ADP + phosphate + L-aminoacyl-L-amino acid
assay at pH 8.0, 30°C
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?
ATP + an L-amino acid + an L-amino acid
ADP + phosphate + L-aminoacyl-L-amino acid
assay at pH 8.0, 30°C
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-
?
ATP + an L-amino acid + an L-amino acid
ADP + phosphate + L-aminoacyl-L-amino acid
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-
?
ATP + an L-amino acid + an L-amino acid
ADP + phosphate + L-aminoacyl-L-amino acid
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-
?
ATP + an L-amino acid + an L-amino acid
ADP + phosphate + L-aminoacyl-L-amino acid
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?
ATP + an L-amino acid + an L-amino acid
ADP + phosphate + L-aminoacyl-L-amino acid
substrate and dipeptide formation specificity analysis, the enzyme shows high activity with L-Phe, L-Met, and L-His, detailed overview. RSp1486a exhibits different substrate specificity from Bacillus subtilus Lal, and preferably synthesizes hetero-dipeptides where a more bulky amino acid is placed at the N-terminus and a less bulky amino acid is placed at the C-terminus in contrast to those synthesized by Bacillus subtilus Lal, overview
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ATP + an L-amino acid + an L-amino acid
ADP + phosphate + L-aminoacyl-L-amino acid
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