EC Number |
Protein Variants |
Reference |
---|
6.3.2.4 | E15Q |
mutant enzyme with negligible depsipeptide synthetase activity. The mutant uniquely activates D-Lac as the electrophilic rather than the nucleophilic partner for condensation with D-Ala to make a regioisomeric D-Lac-D-Ala, an amide rather than an ester product |
707464 |
6.3.2.4 | F261Y |
mutation of the Omega loop, inactive mutant |
728168 |
6.3.2.4 | I16V |
site-directed mutagenesis of an active site mutant, mutant kinetics compared to the wild-type structure |
695037 |
6.3.2.4 | L241F |
site-directed mutagenesis of an active site mutant, mutant kinetics compared to the wild-type structure |
695037 |
6.3.2.4 | L241Y |
site-directed mutagenesis of an active site mutant, mutant kinetics compared to the wild-type structure |
695037 |
6.3.2.4 | L282R |
11fold decrease in kcat for D-alanine, 18fold increase in Km for D-alanine, 23fold decrease in Km for D-serine, 9fold drop in kcat for D-serine |
650819 |
6.3.2.4 | L308T |
site-directed mutagenesis of an active site mutant, mutant kinetics compared to the wild-type structure |
695037 |
6.3.2.4 | more |
9 clinical isolates that show high resistance to vancomycin have mutations in the Ddl gene, lack of growth of these mutants in the absence of vancomycin is most probably due to a complete loss of Ddl activity |
653791 |
6.3.2.4 | more |
homology structure modeling analyses of wild-type enzyme and mutants S293D and S293E |
-, 745494 |
6.3.2.4 | more |
insertion in the middle of the ddl gene is responsible for inactivation of D-alanine-D-alanine ligase in strain BM4416 |
-, 649336 |