Protein Variants | Comment | Organism |
---|---|---|
E15Q | mutant enzyme with negligible depsipeptide synthetase activity. The mutant uniquely activates D-Lac as the electrophilic rather than the nucleophilic partner for condensation with D-Ala to make a regioisomeric D-Lac-D-Ala, an amide rather than an ester product | Escherichia coli |
S150A | mutant has gained depsipeptide (D-Ala-D-Lac, D-Ala-D-hydroxybutyrate) ligase activity with dipeptide/depsipeptide partition ratios that mimic the pH behavior of VanA (D-alanine(R)-lactate ligase) | Escherichia coli |
Y216F | mutant has gained depsipeptide (D-Ala-D-Lac, D-Ala-D-hydroxybutyrate) ligase activity with dipeptide/depsipeptide partition ratios that mimic the pH behavior of VanA (D-alanine(R)-lactate ligase) | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
D-alanine | 37°C, KM-value for D-alanine1 (the first molecule of substrate that is activated by the enzyme molecule) , wild-type enzyme, pH 7.5 | Escherichia coli | |
0.004 | - |
D-alanine | 37°C, KM-value for D-alanine1 (the first molecule of substrate that is activated by the enzyme molecule), wild-type enzyme, pH 9.2 | Escherichia coli | |
0.11 | - |
D-alanine | 37°C, KM-value for D-alanine1 (the first molecule of substrate that is activated by the enzyme molecule), wild-type enzyme, pH 6.0 | Escherichia coli | |
0.51 | - |
D-alanine | 37°C, KM-value for D-alanine1 (the first molecule of substrate that is activated by the enzyme molecule), mutant enzyme E15Q, pH 7.5 | Escherichia coli | |
0.51 | - |
D-alanine | 37°C, KM-value for D-alanine1 (the first molecule of substrate that is activated by the enzyme molecule), mutant enzyme E15Q, pH 9.2 | Escherichia coli | |
0.85 | - |
D-alanine | 37°C, KM-value for D-alanine1 (the first molecule of substrate that is activated by the enzyme molecule), mutant enzyme S150A, pH 7.5 | Escherichia coli | |
1.1 | - |
D-alanine | 37°C, KM-value for D-alanine2 (the second molecule of substrate that is activated by the enzyme molecule), wild-type enzyme, pH 9.2 | Escherichia coli | |
2 | - |
D-alanine | 37°C, KM-value for D-alanine2 (the second molecule of substrate that is activated by the enzyme molecule), wild-type enzyme, pH 7.5 | Escherichia coli | |
4.8 | - |
D-alanine | 37°C, KM-value for D-alanine1 (the first molecule of substrate that is activated by the enzyme molecule), mutant enzyme Y216F, pH 9.2 | Escherichia coli | |
6.2 | - |
D-alanine | 37°C, KM-value for D-alanine1 (the first molecule of substrate that is activated by the enzyme molecule), mutant enzyme S150A, pH 6.0 | Escherichia coli | |
8 | - |
D-alanine | 37°C, KM-value for D-alanine2 (the second molecule of substrate that is activated by the enzyme molecule), wild-type enzyme, pH 6.0 | Escherichia coli | |
11 | - |
D-alanine | 37°C, KM-value for D-alanine1 (the first molecule of substrate that is activated by the enzyme molecule), mutant enzyme E15Q, pH 6.0 | Escherichia coli | |
16 | - |
D-alanine | 37°C, KM-value for D-alanine1 (the first molecule of substrate that is activated by the enzyme molecule), mutant enzyme Y216F, pH 7.5 | Escherichia coli | |
44 | - |
D-alanine | 37°C, KM-value for D-alanine1 (the first molecule of substrate that is activated by the enzyme molecule) , mutant enzyme S150A, pH 9.2 | Escherichia coli | |
65 | - |
D-alanine | 37°C, KM-value for D-alanine2 (the second molecule of substrate that is activated by the enzyme molecule), mutant enzyme E15Q, pH 9.2 | Escherichia coli | |
74 | - |
D-alanine | 37°C, KM-value for D-alanine2 (the second molecule of substrate that is activated by the enzyme molecule), mutant enzyme E15Q, pH 7.5 | Escherichia coli | |
80 | - |
D-alanine | 37°C, KM-value for D-alanine2 (the second molecule of substrate that is activated by the enzyme molecule), mutant enzyme S150A, pH 7.5 | Escherichia coli | |
82 | - |
D-alanine | 37°C, KM-value for D-alanine2 (the second molecule of substrate that is activated by the enzyme molecule), mutant enzyme E15Q, pH 6.0 | Escherichia coli | |
140 | - |
D-alanine | 37°C, KM-value for D-alanine2 (the second molecule of substrate that is activated by the enzyme molecule), mutant enzyme S150A, pH 6.0 | Escherichia coli | |
150 | - |
D-alanine | 37°C, KM-value for D-alanine1 (the first molecule of substrate that is activated by the enzyme molecule) , mutant enzyme Y216F, pH 6.0 | Escherichia coli | |
450 | - |
D-alanine | 37°C, KM-value for D-alanine2 (the second molecule of substrate that is activated by the enzyme molecule), mutant enzyme Y216F, pH 6.0 | Escherichia coli | |
630 | - |
D-alanine | 37°C, KM-value for D-alanine2 (the second molecule of substrate that is activated by the enzyme molecule), mutant enzyme Y216F, pH 7.5 | Escherichia coli | |
1100 | - |
D-alanine | 37°C, KM-value for D-alanine2 (the second molecule of substrate that is activated by the enzyme molecule), mutant enzyme S150A, pH 9.2 | Escherichia coli | |
1200 | - |
D-alanine | 37°C, KM-value for D-alanine2 (the second molecule of substrate that is activated by the enzyme molecule), mutant enzyme Y216F, pH 9.2 | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P07862 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2 D-alanine | Y216, S150, and E15 form a hydrogen-bonding triad that orients an omega-loop to close over the active site and also to orient substrate D-Ala1 (the first molecule of substrate that is activated by the enzyme protein). The bifunctional enzyme also catalyzes the formation of D-alanyl-D-lactate (D-alanine-(R)-lactate ligase) | Escherichia coli | ADP + phosphate + D-alanyl-D-alanine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
D-alanyl-D-alanine ligase B | - |
Escherichia coli |
DdlB | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.3 | - |
D-alanine | 37°C, mutant enzyme E15Q, pH 6.0 | Escherichia coli | |
3.3 | - |
D-alanine | 37°C, mutant enzyme S150A, pH 6.0 | Escherichia coli | |
7.5 | - |
D-alanine | 37°C, mutant enzyme Y216F, pH 6.0 | Escherichia coli | |
10.5 | - |
D-alanine | 37°C, mutant enzyme Y216F, pH 7.5 | Escherichia coli | |
11.83 | - |
D-alanine | 37°C, wild-type enzyme, pH 6.0 | Escherichia coli | |
13.7 | - |
D-alanine | 37°C, mutant enzyme E15Q, pH 7.5 | Escherichia coli | |
14.7 | - |
D-alanine | 37°C, wild-type enzyme, pH 7.5 | Escherichia coli | |
16.3 | - |
D-alanine | 37°C, mutant enzyme S150A, pH 7.5 | Escherichia coli | |
18.3 | - |
D-alanine | 37°C, mutant enzyme E15Q, pH 9.2 | Escherichia coli | |
18.3 | - |
D-alanine | 37°C, mutant enzyme S150A, pH 9.2 | Escherichia coli | |
20 | - |
D-alanine | 37°C, mutant enzyme Y216F, pH 9.2 | Escherichia coli | |
26.7 | - |
D-alanine | 37°C, wild-type enzyme, pH 9.2 | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
D-alanine | 37°C, mutant enzyme S150A, kcat/Km(D-alanine2), pH 6.0. D-Alanine2 is the second molecule of substrate that is activated by the enzyme molecule | Escherichia coli | |
2.4 | - |
D-alanine | 37°C, mutant enzyme E15Q, kcat/Km(D-alanine2), pH 6.0. D-Alanine2 is the second molecule of substrate that is activated by the enzyme molecule | Escherichia coli | |
3 | - |
D-alanine | 37°C, mutant enzyme Y216F, kcat/Km(D-alanine2), pH 6.0. D-Alanine2 is the second molecule of substrate that is activated by the enzyme molecule | Escherichia coli | |
11 | - |
D-alanine | 37°C, mutant enzyme E15Q, kcat/Km(D-alanine2), pH 7.5. D-Alanine2 is the second molecule of substrate that is activated by the enzyme molecule | Escherichia coli | |
12 | - |
D-alanine | 37°C, mutant enzyme S150A, kcat/Km(D-alanine2), pH 7.5. D-Alanine2 is the second molecule of substrate that is activated by the enzyme molecule | Escherichia coli | |
17 | - |
D-alanine | 37°C, mutant enzyme E15Q, kcat/Km(D-alanine2), pH 9.2. D-Alanine2 is the second molecule of substrate that is activated by the enzyme molecule | Escherichia coli | |
26 | - |
D-alanine | 37°C, mutant enzyme S150A, kcat/Km(D-alanine2), pH 9.2. D-Alanine2 is the second molecule of substrate that is activated by the enzyme molecule | Escherichia coli | |
39 | - |
D-alanine | 37°C, mutant enzyme Y216F, kcat/Km(D-alanine2), pH 7.5. D-Alanine2 is the second molecule of substrate that is activated by the enzyme molecule | Escherichia coli | |
89 | - |
D-alanine | 37°C, wild-type enzyme, kcat/Km(D-alanine2), pH 6.0. D-Alanine2 is the second molecule of substrate that is activated by the enzyme molecule | Escherichia coli | |
260 | - |
D-alanine | 37°C, mutant enzyme Y216F, kcat/Km(D-alanine2), pH 9.2. D-Alanine2 is the second molecule of substrate that is activated by the enzyme molecule | Escherichia coli | |
440 | - |
D-alanine | 37°C, wild-type enzyme, kcat/Km(D-alanine2), pH 7.5. D-Alanine2 is the second molecule of substrate that is activated by the enzyme molecule | Escherichia coli | |
1500 | - |
D-alanine | 37°C, wild-type enzyme, kcat/Km(D-alanine2), pH 9.2. D-Alanine2 is the second molecule of substrate that is activated by the enzyme molecule | Escherichia coli |