EC Number   |
Protein Variants |
Reference |
|---|
    6.2.1.13 | E218Dalpha |
1-10% of wild-type activity |
693061 |
| 6.2.1.13 | more |
gradual ACS gene silencing (49-93%) significantly decreases the acetate flux without affecting the levels of glycolytic metabolites and ATP in trophozoites. Amoebae lacking ACS activity are unable to reestablish the acetyl-CoA/CoA ratio after an oxidative stress challenge |
744414 |
| 6.2.1.13 | H257Dalpha |
mutant shows no activity in either direction |
693061 |
| 6.2.1.13 | H252A |
site-directed mutagenesis, alteration of His252 in EhACD effectively eliminate overall enzymatic activity as well as phosphorylation in either direction of the reaction |
744911 |
| 6.2.1.13 | E218alphaQ |
site-directed mutagenesis, comparison of the wild-type CD spectrum with the mutant CD spectrum, structure and reaction kinetics, overview. Inactive mutant, phopshorylation of the mutant is reduced |
693061 |
| 6.2.1.13 | H71betaA |
site-directed mutagenesis, comparison of the wild-type CD spectrum with the mutant CD spectrum, structure and reaction kinetics, overview. Inactive mutant, which is impaired in phosphorylation of the beta subunit |
693061 |
| 6.2.1.13 | H257alphaD |
site-directed mutagenesis, comparison of the wild-type CD spectrum with the mutant CD spectrum, structure and reaction kinetics, overview. Inactive mutant, which is not phosphorylated at both the alpha- and beta-subunit |
693061 |
| 6.2.1.13 | E218alphaD |
site-directed mutagenesis, comparison of the wild-type CD spectrum with the mutant CD spectrum, structure and reaction kinetics, overview. The mutant shows 1-10% of the wild-type activity, phopshorylation of the mutant is reduced |
693061 |
| 6.2.1.13 | D212betaE |
site-directed mutagenesis, comparison of the wild-type CD spectrum with the mutant CD spectrum, structure and reaction kinetics, overview. The mutant shows 2-4% of the wild-type activity, phosphorylation of the mutant is reduced |
693061 |
| 6.2.1.13 | E213A |
site-directed mutagenesis, the mutant variant is not phosphorylated by acetyl-CoA and phosphate |
744911 |
