EC Number   |
Protein Variants |
Reference |
|---|
   5.2.1.8 | A16S |
site-directed mutagenesis, dominant-negative Pin1 point mutation |
705286 |
| 5.2.1.8 | A78G |
52% of wild-type activity |
747407 |
| 5.2.1.8 | A82P |
mutation in hinge region, 41% of wild-type activity. Unlike wild-type, the mutant unfolds by a non-two state mechanism in the presence of urea. Mutation affects both the shape and size of the protein significantly |
747215 |
| 5.2.1.8 | C113A |
isomerase-inactive |
716069 |
| 5.2.1.8 | C113A |
mutation in isomerase domain, catalytically inert. Mutation diminishes binding to brain-specifc protein BNIP-H |
694818 |
| 5.2.1.8 | C113D |
mutation does not compromise isoform Pin1 function in vivo nor does it abolish catalytic activity. C113 may not be the catalytic nucleophile |
681404 |
| 5.2.1.8 | C113S |
catalytically inactive |
715712 |
| 5.2.1.8 | C113S |
decrease in kcat, small in Km value. C113 may not be the catalytic nucleophile |
681404 |
| 5.2.1.8 | C115A |
Cys at position 52, 62, 115, and 161 are mutated individually to Ala and the purified mutant proteins to retain full affinity for cyclosporin A and equivalent catalytic efficiency as a rotamase |
2539 |
| 5.2.1.8 | C129S |
presence of dithiothreitol, 49% of wild-type activity, presence of H2O2, 84% of wild-type activity |
678048 |
