EC Number   |
Protein Variants |
Reference |
|---|
   5.1.3.B12 | A107I |
7.7fold decrease in kcat/Km for D-fructose, no activity detected with D-tagatose |
727220 |
| 5.1.3.B12 | A107P |
1.4fold decrease in kcat/Km for D-fructose, 6.6fold decrease in kcat/Km for D-tagatose |
727220 |
| 5.1.3.B12 | A107V |
13.9fold decrease in kcat/Km for D-fructose, no activity detected with D-tagatose |
727220 |
| 5.1.3.B12 | E150Q |
inactive mutant enzyme |
-, 728138 |
| 5.1.3.B12 | E156D |
mutant exhibits 63% of the wild-type activity |
-, 727221 |
| 5.1.3.B12 | E244Q |
inactive mutant enzyme |
-, 728138 |
| 5.1.3.B12 | G67C |
the variant shows remarkably decreased specific activity |
726718 |
| 5.1.3.B12 | I33E |
the enzyme variant shows no activity |
726718 |
| 5.1.3.B12 | I33K |
the enzyme variant shows no activity |
726718 |
| 5.1.3.B12 | I33L |
increase of 5°C in the temperature for maximal enzyme activity, increase of 7.2-fold in the half-life at 50°C, and increase of 4.3°C in apparent melting temperature, respectively, compared with the wild-type enzyme |
-, 726718 |
