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Literature summary for 5.1.3.B12 extracted from

  • Kim, H.J.; Lim, B.C.; Yeom, S.J.; Kim, Y.S.; Kim, D.; Oh, D.K.
    Roles of Ile66 and Ala107 of D-psicose 3-epimerase from Agrobacterium tumefaciens in binding O6 of its substrate, D-fructose (2009), Biotechnol. Lett., 32, 113-118.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
A107I 7.7fold decrease in kcat/Km for D-fructose, no activity detected with D-tagatose Agrobacterium tumefaciens
A107P 1.4fold decrease in kcat/Km for D-fructose, 6.6fold decrease in kcat/Km for D-tagatose Agrobacterium tumefaciens
A107V 13.9fold decrease in kcat/Km for D-fructose, no activity detected with D-tagatose Agrobacterium tumefaciens
I66A 1.4fold increase in kcat/Km for D-fructose, 150fold decrease in kcat/Km for D-tagatose Agrobacterium tumefaciens
I66C 3fold decrease in kcat/Km for D-fructose, 29.2fold decrease in kcat/Km for D-tagatose Agrobacterium tumefaciens
I66F 2.1fold decrease in kcat/Km for D-fructose, 23.3fold decrease in kcat/Km for D-tagatose Agrobacterium tumefaciens
I66G 1.9fold decrease in kcat/Km for D-fructose, 87.5fold decrease in kcat/Km for D-tagatose Agrobacterium tumefaciens
I66L 1.6fold increase in kcat/Km for D-fructose, 58,3fold decrease in kcat/Km for D-tagatose Agrobacterium tumefaciens
I66V 1.9fold decrease in kcat/Km for D-fructose, 24fold decrease in kcat/Km for D-tagatose Agrobacterium tumefaciens
I66W 1.4fold decrease in kcat/Km for D-fructose, 42fold decrease in kcat/Km for D-tagatose Agrobacterium tumefaciens
I66Y 3fold decrease in kcat/Km for D-fructose, 26fold decrease in kcat/Km for D-tagatose Agrobacterium tumefaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1 7 D-fructose pH 8.0, 45°C, mutant enzyme I66V Agrobacterium tumefaciens
3 5 D-fructose pH 8.0, 45°C, mutant enzyme I66F Agrobacterium tumefaciens
6 11 D-tagatose pH 8.0, 45°C, mutant enzyme I66C Agrobacterium tumefaciens
11
-
D-fructose pH 8.0, 45°C, mutant enzyme I66L Agrobacterium tumefaciens
16
-
D-fructose pH 8.0, 45°C, mutant enzyme I66W Agrobacterium tumefaciens
19
-
D-fructose pH 8.0, 45°C, mutant enzyme I66C Agrobacterium tumefaciens
22
-
D-fructose pH 8.0, 45°C, mutant enzyme I66A Agrobacterium tumefaciens
22
-
D-fructose pH 8.0, 45°C, mutant enzyme I66Y Agrobacterium tumefaciens
28
-
D-fructose pH 8.0, 45°C, mutant enzyme I66G Agrobacterium tumefaciens
33
-
D-fructose pH 8.0, 45°C, wild-type enzyme Agrobacterium tumefaciens
36
-
D-fructose pH 8.0, 45°C, mutant enzyme A107V Agrobacterium tumefaciens
67
-
D-fructose pH 8.0, 45°C, mutant enzyme A107I Agrobacterium tumefaciens
91
-
D-tagatose pH 8.0, 45°C, wild-type enzyme Agrobacterium tumefaciens
140
-
D-fructose pH 8.0, 45°C, mutant enzyme A107P Agrobacterium tumefaciens
213
-
D-tagatose pH 8.0, 45°C, mutant enzyme I66W Agrobacterium tumefaciens
429
-
D-tagatose pH 8.0, 45°C, mutant enzyme A107P Agrobacterium tumefaciens
694
-
D-tagatose pH 8.0, 45°C, mutant enzyme I66F Agrobacterium tumefaciens
696
-
D-tagatose pH 8.0, 45°C, mutant enzyme I66Y Agrobacterium tumefaciens
1146
-
D-tagatose pH 8.0, 45°C, mutant enzyme I66V Agrobacterium tumefaciens
1369
-
D-tagatose pH 8.0, 45°C, mutant enzyme I66G Agrobacterium tumefaciens
1408
-
D-tagatose pH 8.0, 45°C, mutant enzyme I66L Agrobacterium tumefaciens
1640
-
D-tagatose pH 8.0, 45°C, mutant enzyme I66A Agrobacterium tumefaciens

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens A9CH28
-
-
Agrobacterium tumefaciens ATCC 33970 A9CH28
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Agrobacterium tumefaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose
-
Agrobacterium tumefaciens D-psicose
-
r
D-fructose
-
Agrobacterium tumefaciens ATCC 33970 D-psicose
-
r
D-psicose
-
Agrobacterium tumefaciens D-fructose
-
r
D-psicose
-
Agrobacterium tumefaciens ATCC 33970 D-fructose
-
r
D-sorbose
-
Agrobacterium tumefaciens D-tagatose
-
r
D-sorbose
-
Agrobacterium tumefaciens ATCC 33970 D-tagatose
-
r
D-tagatose
-
Agrobacterium tumefaciens D-sorbose
-
r
D-tagatose
-
Agrobacterium tumefaciens ATCC 33970 D-sorbose
-
r

Synonyms

Synonyms Comment Organism
Atu4750 locus name Agrobacterium tumefaciens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
assay at Agrobacterium tumefaciens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
11
-
D-tagatose pH 8.0, 45°C, mutant enzyme I66W Agrobacterium tumefaciens
23
-
D-tagatose pH 8.0, 45°C, mutant enzyme I66A Agrobacterium tumefaciens
26
-
D-fructose pH 8.0, 45°C, mutant enzyme A107V Agrobacterium tumefaciens
33
-
D-tagatose pH 8.0, 45°C, mutant enzyme I66G Agrobacterium tumefaciens
44
-
D-tagatose pH 8.0, 45°C, mutant enzyme I66C Agrobacterium tumefaciens
50
-
D-tagatose pH 8.0, 45°C, mutant enzyme I66L Agrobacterium tumefaciens
56
-
D-tagatose pH 8.0, 45°C, mutant enzyme I66Y Agrobacterium tumefaciens
62
-
D-fructose pH 8.0, 45°C, mutant enzyme I66C Agrobacterium tumefaciens
64
-
D-tagatose pH 8.0, 45°C, mutant enzyme I66F Agrobacterium tumefaciens
72
-
D-fructose pH 8.0, 45°C, mutant enzyme I66Y Agrobacterium tumefaciens
88
-
D-fructose pH 8.0, 45°C, mutant enzyme A107I Agrobacterium tumefaciens
88
-
D-fructose pH 8.0, 45°C, mutant enzyme I66V Agrobacterium tumefaciens
100
-
D-tagatose pH 8.0, 45°C, mutant enzyme I66V Agrobacterium tumefaciens
116
-
D-fructose pH 8.0, 45°C, mutant enzyme I66W Agrobacterium tumefaciens
136
-
D-tagatose pH 8.0, 45°C, mutant enzyme A107P Agrobacterium tumefaciens
152
-
D-fructose pH 8.0, 45°C, mutant enzyme I66G Agrobacterium tumefaciens
168
-
D-fructose pH 8.0, 45°C, mutant enzyme I66F Agrobacterium tumefaciens
176
-
D-fructose pH 8.0, 45°C, mutant enzyme I66L Agrobacterium tumefaciens
194
-
D-tagatose pH 8.0, 45°C, wild-type enzyme Agrobacterium tumefaciens
308
-
D-fructose pH 8.0, 45°C, mutant enzyme I66A Agrobacterium tumefaciens
341
-
D-fructose pH 8.0, 45°C, wild-type enzyme Agrobacterium tumefaciens
996
-
D-fructose pH 8.0, 45°C, mutant enzyme A107P Agrobacterium tumefaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Agrobacterium tumefaciens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.014
-
D-tagatose pH 8.0, 45°C, mutant enzyme I66A Agrobacterium tumefaciens
0.024
-
D-tagatose pH 8.0, 45°C, mutant enzyme I66G Agrobacterium tumefaciens
0.036
-
D-tagatose pH 8.0, 45°C, mutant enzyme I66L Agrobacterium tumefaciens
0.05
-
D-tagatose pH 8.0, 45°C, mutant enzyme I66W Agrobacterium tumefaciens
0.072
-
D-tagatose pH 8.0, 45°C, mutant enzyme I66C Agrobacterium tumefaciens
0.08
-
D-tagatose pH 8.0, 45°C, mutant enzyme I66Y Agrobacterium tumefaciens
0.087
-
D-tagatose pH 8.0, 45°C, mutant enzyme I66V Agrobacterium tumefaciens
0.09
-
D-tagatose pH 8.0, 45°C, mutant enzyme I66F Agrobacterium tumefaciens
0.32
-
D-tagatose pH 8.0, 45°C, mutant enzyme A107P Agrobacterium tumefaciens
0.72
-
D-fructose pH 8.0, 45°C, mutant enzyme A107V Agrobacterium tumefaciens
1.3
-
D-fructose pH 8.0, 45°C, mutant enzyme A107I Agrobacterium tumefaciens
2.1
-
D-tagatose pH 8.0, 45°C, wild-type enzyme Agrobacterium tumefaciens
3.3
-
D-fructose pH 8.0, 45°C, mutant enzyme I66C Agrobacterium tumefaciens
3.3
-
D-fructose pH 8.0, 45°C, mutant enzyme I66Y Agrobacterium tumefaciens
4.8
-
D-fructose pH 8.0, 45°C, mutant enzyme I66F Agrobacterium tumefaciens
5.2
-
D-fructose pH 8.0, 45°C, mutant enzyme I66V Agrobacterium tumefaciens
5.4
-
D-fructose pH 8.0, 45°C, mutant enzyme I66G Agrobacterium tumefaciens
7
-
D-fructose pH 8.0, 45°C, mutant enzyme A107P Agrobacterium tumefaciens
7.3
-
D-fructose pH 8.0, 45°C, mutant enzyme I66W Agrobacterium tumefaciens
10
-
D-fructose pH 8.0, 45°C, wild-type enzyme Agrobacterium tumefaciens
14
-
D-fructose pH 8.0, 45°C, mutant enzyme I66A Agrobacterium tumefaciens
16
-
D-fructose pH 8.0, 45°C, mutant enzyme I66L Agrobacterium tumefaciens