EC Number   |
Protein Variants |
Reference |
|---|
   4.5.1.3 | H8F |
little effect on the activity, but slower turnover of dibromomethane than wild-type |
-, 648762 |
| 4.5.1.3 | H8L |
little effect on the activity, but slower turnover of dibromomethane than wild-type |
-, 648762 |
| 4.5.1.3 | H8Y |
little effect on the activity, but slower turnover of dibromomethane than wild-type |
648762 |
| 4.5.1.3 | more |
generation of a METDI2657 gene knockout mutant strain NOK353 from Methylobacterium dichloromethanicum wild-type strain DM4 using the mobilized suicide vector pK18mob, the mutant strain shows reduced growth on methanol compared to the wild-type enzyme |
730345 |
| 4.5.1.3 | S12A |
drastically reduced activity |
-, 648762 |
| 4.5.1.3 | S12T |
drastically reduced activity |
648762 |
| 4.5.1.3 | V18A |
lower affinity for GSH and slower turnover of dichlormethane than wild-type |
-, 648761 |
| 4.5.1.3 | V18I |
lower affinity for GSH and slower turnover of dichlormethane than wild-type |
-, 648761 |
| 4.5.1.3 | V18T |
lower affinity for GSH and slower turnover of dichlormethane than wild-type |
-, 648761 |
| 4.5.1.3 | W117Y |
higher KM and lower specific activity for all tested substrates, better heat stability than wild-type |
-, 648763 |
