Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.5.1.3 extracted from

  • Vuilleumier, S.; Leisinger, T.
    Protein engineering studies of dichloromethane dehalogenase/glutathione S-transferase from Methylophilus sp. strain DM11. Ser12 but not Tyr6 is required for enzyme activity (1996), Eur. J. Biochem., 239, 410-417.
    View publication on PubMed
Search for more literature for 4.5.1.3

Activating Compound

Activating Compound Comment Organism Structure
glutathione
-
 Methylophilus sp.

Cloned(Commentary)

Cloned (Comment) Organism
-
 Methylophilus sp.

Protein Variants

Protein Variants Comment Organism
H8F little effect on the activity, but slower turnover of dibromomethane than wild-type Methylophilus sp.
H8L little effect on the activity, but slower turnover of dibromomethane than wild-type Methylophilus sp.
H8Y little effect on the activity, but slower turnover of dibromomethane than wild-type Methylophilus sp.
S12A drastically reduced activity Methylophilus sp.
S12T drastically reduced activity Methylophilus sp.
Y6A little effect on the activity Methylophilus sp.
Y6F little effect on the activity Methylophilus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0005
-
 Dibromomethane 30°C, pH 8.5, Y6A Methylophilus sp.
0.001
-
 Dibromomethane 30°C, pH 8.5, H8F Methylophilus sp.
0.0029
-
 Dibromomethane 30°C, pH 8.5, Y6F Methylophilus sp.
0.0032
-
 Dibromomethane 30°C, pH 8.5, H8Y Methylophilus sp.
0.0035
-
 Dibromomethane 30°C, pH 8.5, wild-type Methylophilus sp.
0.032
-
 Dichloromethane 30°C, pH 8.5, Y6F Methylophilus sp.
0.034
-
 Dichloromethane 30°C, pH 8.5, Y6A Methylophilus sp.
0.049
-
 Dichloromethane 30°C, pH 8.5, H8F Methylophilus sp.
0.055
-
 Dichloromethane 30°C, pH 8.5, H8Y Methylophilus sp.
0.059
-
 Dichloromethane 30°C, pH 8.5, wild-type Methylophilus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dichloromethane + H2O Methylophilus sp.
-
 formaldehyde + Cl-
-
 ?
dichloromethane + H2O Methylophilus sp. DM11
-
 formaldehyde + Cl-
-
 ?

Organism

Organism UniProt Comment Textmining
Methylophilus sp. P43387
-
-
Methylophilus sp. DM11 P43387
-
-

Purification (Commentary)

Purification (Comment) Organism
strain DM11 Methylophilus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dibromomethane + H2O
-
 Methylophilus sp. formaldehyde + Br-
-
 ?
dibromomethane + H2O
-
 Methylophilus sp. DM11 formaldehyde + Br-
-
 ?
dichloromethane + H2O
-
 Methylophilus sp. formaldehyde + Cl-
-
 ?
dichloromethane + H2O
-
 Methylophilus sp. DM11 formaldehyde + Cl-
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3
-
 Dichloromethane 30°C, pH 8.5, Y6A Methylophilus sp.
2.6
-
 Dichloromethane 30°C, pH 8.5, Y6F Methylophilus sp.
3
-
 Dichloromethane 30°C, pH 8.5, H8Y Methylophilus sp.
3
-
 Dibromomethane 30°C, pH 8.5, H8Y Methylophilus sp.
3.3
-
 Dichloromethane 30°C, pH 8.5, wild-type Methylophilus sp.
3.4
-
 Dibromomethane 30°C, pH 8.5, H8F Methylophilus sp.
3.6
-
 Dichloromethane 30°C, pH 8.5, H8F Methylophilus sp.
4.4
-
 Dibromomethane 30°C, pH 8.5, Y6A Methylophilus sp.
5.5
-
 Dibromomethane 30°C, pH 8.5, wild-type Methylophilus sp.
5.6
-
 Dibromomethane 30°C, pH 8.5, Y6F Methylophilus sp.