EC Number   |
Protein Variants |
Reference |
|---|
    4.2.1.84 | A51L |
wild-type enzyme catalyzes the conversion of (S)-mandelamide with an enantiomeric excess of 52.6%, the beta-subunit mutant enzyme catalyzes the reaction with an enantiomeric excess of 74.3% |
-, 747347 |
| 4.2.1.84 | alphaD164N |
site-directed mutagenesis |
-, 714254 |
| 4.2.1.84 | alphaE168Q |
site-directed mutagenesis |
-, 714254 |
| 4.2.1.84 | alphaR170Q |
site-directed mutagenesis |
-, 714254 |
| 4.2.1.84 | alphaT109S |
site-directed mutagenesis, the mutant shows similar characteristics compared to the wild-type enzyme |
-, 648457 |
| 4.2.1.84 | alphaV5L |
site-directed mutagenesis, exchange in the H-NHase does not influence the catalytic activity or the Co2+ content |
-, 714229 |
| 4.2.1.84 | alphaY114T |
site-directed mutagenesis, the mutant shows a very low cobalt content and catalytic activity compared to the wild-type enzyme, and oxidative modifications of aCys111 and aCys113 residues are not observed |
-, 648457 |
| 4.2.1.84 | betaE56Q |
site-directed mutagenesis |
-, 714254 |
| 4.2.1.84 | betaH71F |
site-directed mutagenesis |
714254 |
| 4.2.1.84 | betaH71L |
site-directed mutagenesis |
-, 714254 |
