EC Number |
Protein Variants |
Reference |
---|
4.2.1.78 | A79F |
improved turnover of ketones, increase in side-chain steric interactions |
748745 |
4.2.1.78 | A79I |
improved turnover of ketones, increase in side-chain steric interactions |
748745 |
4.2.1.78 | E110A |
site-directed mutagenesis, lower but significant activity |
693259 |
4.2.1.78 | K122A |
site-directed mutagenesis, lower but significant activity |
693259 |
4.2.1.78 | more |
virus-induced gene silencing of NCS in opium poppy profoundly reduces alkaloid levels compared to controls, a mixed culture of Agrobacterium tumefaciens harboring either pTRV2-PsNCS or pTRV1 is infiltrated into opium poppy seedlings tow weeks after germination. Levels of the five major alkaloids in opium poppy latex, i.e. morphine, codeine, thebaine, noscapine, and papaveine, are reduced by 75-82% in plants infiltrated with Agrobacterium tumefaciens harboring pTRV2-PsNCS |
716518 |
4.2.1.78 | Y108A |
site-directed mutagenesis abolishes the stereoselective synthesis of (S)-norcoclaurine |
693259 |
4.2.1.78 | Y108F |
site-directed mutagenesis, reduced activity |
693259 |