EC Number |
Protein Variants |
Reference |
---|
4.2.1.3 | C381A |
site-directed mutagenesis |
-, 715933 |
4.2.1.3 | C447A |
site-directed mutagenesis |
-, 715933 |
4.2.1.3 | C450S |
mutant is enzymatically inactive, glutamate auxotroph and accumulates citrate. Mutant strain exhibits overexpression of the citB promoter and accumulates high levels of aconitase protein. Mutant strain exhibits increased levels of citrate synthase protein. Mutant enzyme does not bind to the citrate synthase 5' leader RNA in vitro |
729925 |
4.2.1.3 | C459S |
catalytically inactive mutant without its [4Fe-4S]-cluster. Mitochondrial morphological defects as a consequence of acon inactivation depend on its [4Fe-4S] cluster |
730672 |
4.2.1.3 | C517A |
enzymatically inactive mutant enzyme that still binds iron responsive elements |
653617 |
4.2.1.3 | C538A |
mutation of cysteine residue involved in the coordination of the [4Fe-4S] cluster, mutant shows no catalytic activity. Mutant displays a lower affinity for the IRE sequence than the wild-type aconitase as shown in gel shift assays |
729645 |
4.2.1.3 | DELTA125-129 |
mutant shows lower activity compared to wild-type |
729645 |
4.2.1.3 | H170A |
site-directed mutagenesis |
-, 715933 |
4.2.1.3 | more |
construction of a fusion protein of aconitase B and isocitrate dehydrogenase, ICDH and AcnB, i.e. ICDH-AcnB, structure determination of ICDH-AcnB, overview |
696416 |
4.2.1.3 | more |
construction of diverse deletion mutants of aconitase, overview. Deletion of six C-terminal amino acids does not eliminate enzymatic activity but abolishes dual targeting |
-, 715933 |