EC Number   |
Protein Variants |
Reference |
|---|
    4.2.1.130 | C111A |
site-directed mutagenesis, catalytically inactive mutant |
729499 |
| 4.2.1.130 | C185A |
mutation almost completely abolishes glyoxalase activity |
716271 |
| 4.2.1.130 | C190A |
diminished glyoxalase III activity |
-, 753036 |
| 4.2.1.130 | E16A |
site-directed mutagenesis, catalytically inactive mutant |
729499 |
| 4.2.1.130 | E77A |
mutation almost completely abolishes glyoxalase activity |
716271 |
| 4.2.1.130 | E81A |
diminished glyoxalase III activity |
-, 753036 |
| 4.2.1.130 | H130A |
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme |
729499 |
| 4.2.1.130 | H184A |
kcat/KM is 3.6fold lower compared to the wild-type value |
716271 |
| 4.2.1.130 | H186A |
mutant enzyme shows approximately 17% remaining activity |
716271 |
| 4.2.1.130 | H191A |
marginal glyoxalase III activity |
-, 753036 |
