EC Number   |
Protein Variants |
Reference |
|---|
    4.1.99.12 | more |
an additional single copy of the ribA gene introduced into the sacBlocus of the riboflavin production strain and constitutive expression from the medium strength vegI promoter leads to improved riboflavin titers and yields of riboflavin on glucose of up to 25%, strain VB2XL1, both enzymatic activities of RibA, the 3,4-dihydroxy-2-butanone 4-phosphate synthase activity located in the N-terminal half of the protein and the GTP cyclohydrolase II activity of the C-terminal domain, are necessary for the improved riboflavin productivity, method, overview |
675226 |
| 4.1.99.12 | more |
construction of RIB3 disruption mutants, restoration by riboflavin of growth of a rib3 deletion mutant on glucose but not glycerol/ethanol, phenotype, overview |
674407 |
| 4.1.99.12 | E154D |
DBPS activity is abolished |
716533 |
| 4.1.99.12 | more |
engineering of Escherichia coli for increased riboflavin production: overexpression of gene ribB to increase the flux from ribulose 5-phosphate to 3,4-dihydroxybutan-2-one 4-phosphate. Then ndk and gmk genes are overexpressed to enhance GTP supply. Subsequently, a R419L mutation is introduced into purA to reduce the flux from IMP to AMP. Co-overexpression of mutant purF and prs genes further increased riboflavin production. The final strain RF18S produces 387.6 mg riboflavin per liter with a yield of 44.8 mg riboflavin per gram glucose in shake-flask fermentations. The final titer and yield are 72.2% and 55.6%. Mutant strain and method evaluation, overview |
-, 747595 |
| 4.1.99.12 | A137T |
naturally occuring Rib3 mutant which is partially deficient in cytochromes a, a3, and cytochrome b, the respiratory defect elicited by this mutation cannot be explained by a flavin insufficiency based on the following evidence: 1. growth of the aE280/U1 on respiratory substrates is not rescued by exogenous riboflavin, 2. the levels of flavin nucleotides are not significantly different in the mutant and wild type, phenotype, overview |
674407 |
| 4.1.99.12 | Q181R/Q183R |
site-directed mutagenesis, construction of a synthetic gene, derived from orf 6.2440, with nucleotide exchanges at positions 414, 426, 477, 480, and 581 |
675342 |
| 4.1.99.12 | D21E |
site-directed mutagenesis, inactive mutant |
674406 |
| 4.1.99.12 | D21N |
site-directed mutagenesis, inactive mutant |
674406 |
| 4.1.99.12 | D30E |
site-directed mutagenesis, inactive mutant |
674406 |
| 4.1.99.12 | D92A |
site-directed mutagenesis, inactive mutant |
675342 |
