EC Number   |
Protein Variants |
Reference |
|---|
    4.1.3.1 | A214S |
increased thermostability and low specific activity at low temperature |
-, 679717 |
| 4.1.3.1 | A219C |
similar catalytic properties as the wild-type enzyme |
649253 |
| 4.1.3.1 | A231E |
more thermolabile than the wild type enzyme |
-, 679717 |
| 4.1.3.1 | A341N |
decreased activity compared to the wild type enzyme |
-, 679717 |
| 4.1.3.1 | C144A |
mutant with no enzyme activity |
664083 |
| 4.1.3.1 | C165S |
behaves as the wild-type |
704605 |
| 4.1.3.1 | C165S/C247S |
is inactivated by 5 mM GSSG and reactivated by treatment with either 20 mM reduced dithiothreitol or reduced glutaredoxin 1 as the wild-type protein. Complete shift in molecular mass corresponding to one glutathione adduct after 5 h of incubation, but prolonged incubation does not induce any additional glutathionylation |
704605 |
| 4.1.3.1 | C165S/C247S/C301S |
is inactivated by 5 mM GSSG and reactivated by treatment with either 20 mM reduced dithiothreitol or reduced glutaredoxin 1 as the wild-type protein. Complete shift in molecular mass corresponding to one glutathione adduct after 5 h of incubation, but prolonged incubation does not induce any additional glutathionylation |
704605 |
| 4.1.3.1 | C178S |
is totally inactive |
704605 |
| 4.1.3.1 | C191A |
unable to form the closed conformation crystal form |
-, 681995 |
