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EC Number
Protein Variants
Commentary
Reference
4.1.2.61
E143A
Glu143 is essential for catalysis
-
,
690831
4.1.2.61
S123A
mutation of Ser123 to alanine results in a mutant displaying a reduced turnover nummber compared with the wild-type and a KM comparable with that of the wild-type, suggesting that there is little contribution of this residue to mechanism or substrate binding
-
,
690831
4.1.2.61
S123A/E143A
no activity
-
,
690831
4.1.2.61
Y239F
Tyr239 is an important residue in determining substrate specificity
-
,
690831
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