EC Number   |
Protein Variants |
Reference |
|---|
    4.1.1.49 | D241A |
complete loss of activity |
746914 |
| 4.1.1.49 | D242A |
complete loss of activity |
746914 |
| 4.1.1.49 | D263E |
16800fold reduction in maximal velocity compared to wild-type enzyme, KM-value for ADP is 1.8fold lower than wild-type value, KM-value for phosphoenolpyruvate is 1.3fold higher than wild-type value, KM-value for HCO3- is 1.1fold lower than wild-type value |
661280 |
| 4.1.1.49 | D269E |
site-specific mutagenesis |
651719 |
| 4.1.1.49 | deltaF409 |
site-specific mutagenesis, deletion of Phe409 |
651719 |
| 4.1.1.49 | E272Q |
the mutation increases the activation constant for Mn2+ in the main reaction of the enzyme 11fold |
702488 |
| 4.1.1.49 | E284Q |
the mutation increases the activation constant for Mn2+ in the main reaction of the enzyme 69fold, the mutation changes the nucleotide-independent oxalacetate decarboxylase activity of PEP carboxykinase into an ADP-requiring activity |
702488 |
| 4.1.1.49 | E299A |
the mutation has no effect on catalytic efficiency |
716840 |
| 4.1.1.49 | E508Q/E511Q |
site-specific mutagenesis |
651719 |
| 4.1.1.49 | F216Y |
kinetic parameter in the PEP carboxylation direction: Km: 0.170 mM (MnADP-), 0.917 mM (phosphoenolpyruvate), 0.056 mM (Mn2+), 0.024 mM (HCO3-) |
678490 |
