EC Number   |
Protein Variants |
Reference |
|---|
    4.1.1.22 | C179S/C417S |
double mutation prevents nonspecific polymerization and improves the homogeneity of purified enzyme |
726619 |
| 4.1.1.22 | C180S/C418S |
mutation facilitates the purification and crystallization of enzyme. Mutant shows Km and kcat values similar to wild-type |
727920 |
| 4.1.1.22 | C316V |
the mutant shows 34% activity compared to the wild type enzyme |
715728 |
| 4.1.1.22 | C552A |
site-directed mutagenesis, the mutant shows similar cleavage by caspase-9 as the wild-type enzyme |
680763 |
| 4.1.1.22 | D271E |
mutant conserves 2% of the wild type HDC activity |
693044 |
| 4.1.1.22 | D315N |
no enzymatic activity |
715728 |
| 4.1.1.22 | D315V |
no enzymatic activity |
715728 |
| 4.1.1.22 | D517A |
site-directed mutagenesis, the mutant shows reduced cleavage by caspase-9 compared to the wild-type enzyme |
-, 680763 |
| 4.1.1.22 | D518A |
site-directed mutagenesis, the mutant shows reduced cleavage by caspase-9 compared to the wild-type enzyme |
-, 680763 |
| 4.1.1.22 | D518A/D550A/D551A |
site-directed mutagenesis, the mutant shows highly reduced activation by butyrate compared to the wild-type enzyme |
680763 |
