EC Number   |
Protein Variants |
Reference |
|---|
   3.6.5.3 | A208V |
suppressor mutation to rescue growth defect associated with N135D mutation |
-, 700161 |
| 3.6.5.3 | A219T |
suppressor mutation to rescue growth defect associated with N135D mutation, as single mutant slow-growth phenotype |
700161 |
| 3.6.5.3 | A26G |
converts the guanine nucleotide binding consensus sequences A-X-X-X-X-G-K-[T,S] of the elongation factor EF-2 into the corresponding G-X-X-X-X-G-K-[T,S] motif which is present in all the other GTP-binding proteins. In the mutant, the rate of poly(U)-directed poly(Phe) synthesis and the ribosome-dependent GTPase activity of A26GSsEF-2 are decreased. A26G substitution enhances the catalytic efficiency of the intrinsic SsEF-2 GTPase triggered by ethylene glycol and decreases the affinity for GDP |
679626 |
| 3.6.5.3 | A26G |
the mutant of isoform EF-2 shows increased wild type activity |
740115 |
| 3.6.5.3 | A375T |
resistance to kirromycin, abolished streptomycin resistance of mutants of ribosomal protein S12 |
-, 701199 |
| 3.6.5.3 | A382V |
suppressor mutation to rescue growth defect associated with N135D mutation |
-, 700161 |
| 3.6.5.3 | A421(insGly)G422 |
mutation causes cold-sensitivity in the organism. No GTPase activity below 10°C and reduced activity at all temperatures up to 45°C, as compared to wild-type enzyme |
659697 |
| 3.6.5.3 | D138N |
mutant with decreased affinity for GDP and increased affinity for XDP |
-, 644150 |
| 3.6.5.3 | D19A |
site-directed mutagenesis, the mutation does not strongly modify the GDP binding properties of the two mutant enzyme, but reduces the GTP hydrolysis rate |
-, 757829 |
| 3.6.5.3 | D19A/H97A |
site-directed mutagenesis, almost inactive mutant |
-, 757829 |
