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Literature summary for 3.6.5.3 extracted from

  • Bocchini, V.; Adinolfi, B.S.; Arcari, P.; Arcucci, A.; Dello Russo, A.; De Vendittis, E.; Ianniciello, G.; Masullo, M.; Raimo, G.
    Protein engineering on enzymes of the peptide elongation cycle in Sulfolobus solfataricus (1998), Biochimie, 80, 895-898.
    View publication on PubMed
Search for more literature for 3.6.5.3

Activating Compound

Activating Compound Comment Organism Structure
NaCl
-
 Saccharolobus solfataricus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
A26G the mutant of isoform EF-2 shows increased wild type activity Saccharolobus solfataricus
Y54H the mutant of isoform EF-1beta shows wild type activity Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
-
 Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Saccharolobus solfataricus
-
 GDP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus P30925
-
-
Saccharolobus solfataricus P35021
-
-
Saccharolobus solfataricus Q64214
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
 Saccharolobus solfataricus GDP + phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 49000, isoform EF-1alpha, calculated from amino acid sequence Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
EF-1alpha
-
 Saccharolobus solfataricus
EF-1beta
-
 Saccharolobus solfataricus
EF-2
-
 Saccharolobus solfataricus
elongation factor-1alpha
-
 Saccharolobus solfataricus
elongation factor-1beta
-
 Saccharolobus solfataricus
elongation factor-2
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
-
 Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
87
-
 the enzyme isoform EF-1alpha retains 54% activity after 6 h at 87°C Saccharolobus solfataricus
105
-
 the enzyme isoform EF-1beta retains 50% activity after 30 min at 105°C Saccharolobus solfataricus

pI Value

Organism Comment pI Value Maximum pI Value
Saccharolobus solfataricus isoform EF-1alpha, calculated from amino acid sequence
-
 9.1