EC Number |
Protein Variants |
Reference |
---|
3.5.4.5 | C53H |
attempts to express and purify the mutant enzyme are unsuccessful |
654757 |
3.5.4.5 | C53H/R56Q |
mutant enzyme contains the same amount of zinc as the wild-type enzyme. Because of dissociation into its inactive subunits, it is impossible to determine the kinetic parameters for the mutant enzyme |
654757 |
3.5.4.5 | E47A |
mutant protein is insoluble |
-, 755377 |
3.5.4.5 | E47D |
37fold decrease in kcat value. No kcat and kcat/KM dependence on pH values ranging from 4.0 to 11 is observed |
-, 755377 |
3.5.4.5 | E47H |
mutant protein is insoluble |
-, 755377 |
3.5.4.5 | E47L |
complete loss of activity |
-, 755377 |
3.5.4.5 | E47Q |
19fold decrease in kcat value |
-, 755377 |
3.5.4.5 | F137A |
site-directed mutagenesis, inactive monomeric enzyme |
689935 |
3.5.4.5 | F137W/W113F |
5-fluorozebularine does not protect mutant enzyme from dissociation by SDS as it does protect the wild-type enzyme |
657227 |
3.5.4.5 | R56A |
mutant enzyme contains the same amount of zinc as the wild-type enzyme. Similar KM-value but decreased Vmax-value compared to wild-type enzyme |
654757 |