EC Number |
Protein Variants |
Reference |
---|
3.5.4.42 | D188A |
about 6fold increase in kcat/Km value |
739428 |
3.5.4.42 | D188N |
activity similar to wild-type |
739428 |
3.5.4.42 | D303A |
activity similar to wild-type |
739428 |
3.5.4.42 | D303N |
activity similar to wild-type |
739428 |
3.5.4.42 | H219A |
4fold increase in kcat value |
739428 |
3.5.4.42 | H249A |
substitution of an active site histidine residue, complete loss of activity |
739428 |
3.5.4.42 | K65A |
more than 15fold increase in kcat/Km value, 30fold increase in kcat for ammelide |
739428 |
3.5.4.42 | K65R |
more than 15fold increase in kcat/Km value, 12fold increase in kcat for ammelide |
739428 |
3.5.4.42 | N304A |
slight increase in kcat for ammelide |
739428 |
3.5.4.42 | N304D |
7fold increase in kcat for ammelide |
739428 |