EC Number   |
Protein Variants |
Reference |
|---|
   3.5.1.46 | D181H |
- |
-, 172041 |
| 3.5.1.46 | D181K |
- |
-, 172041 |
| 3.5.1.46 | T3A/P4R/T5S/S8Q/D15G |
construction of a hybrid of isozymes EII and EII', termed Hyb24, by five amino acid replacement in EII', the mutant shows the same activity as EII' |
667074, 669406 |
| 3.5.1.46 | more |
construction of chimeric NylB/NylB' mutants Hyb-24DN and Hyb-24DNY. A NylB/NylB' hybrid enzyme Hyb-24 (NylB' containing T3A-P4R-T5S-S8Q-D15G substitutions) has about 0.5% of the NylB level of Ald-hydrolytic activity. Surface structure of the entrance of the catalytic cleft of the Hyb-24DNY and its mutant enzymes, wild-type and mutant kinetics, detailed overview |
753567 |
| 3.5.1.46 | G181D/H266N |
construction of NylB mutant enzyme Hyb-24DN, the activity in Hyb-24 is enhanced 150fold by the two substitutions, where Asp181-COO- stabilizes the substrate binding by electrostatic interactions with Ald-NH3+, and Asn266 cooperatively improves the electrostatic environment with Asp181 |
753567 |
| 3.5.1.46 | D181E |
mutant with increased Km and decreased activity |
-, 172041 |
| 3.5.1.46 | D181N |
mutant with increased Km and decreased activity |
-, 172041 |
| 3.5.1.46 | A61V/A124V/R187S/F264C/G291R/G338A/D370Y |
site-directed mutagenesis |
753567 |
| 3.5.1.46 | D181K |
site-directed mutagenesis of EII, nearly inactive mutant |
669406 |
| 3.5.1.46 | D181H |
site-directed mutagenesis of EII, the mutant shows highly reduced activity compared to the wild-type enzyme |
669406 |
