EC Number   |
Protein Variants |
Reference  |
|---|
   3.4.21.97 | H47A |
inactive mutant, active if coexpressed with AW5 which encodes the first 179 amino acids of assemblin with the addition of Ile, Gln, Thr |
95597 |
| 3.4.21.97 | S118A |
inactive mutant, active if coexpressed with AW5 which encodes the first 179 amino acids of assemblin with the addition of Ile, Gln, Thr |
95597 |
| 3.4.21.97 | A134Q |
oligomerization at high protein concentration |
95600 |
| 3.4.21.97 | A133V |
I-site mutant |
95603 |
| 3.4.21.97 | S132A |
active-site mutant |
95603 |
| 3.4.21.97 | V141G/V207G |
soluble and stable, indistinguishable from wild-type in vitro |
95604 |
| 3.4.21.97 | A143Q/H157A |
the ratio of turnover number to Km-value is 22fold lower than that of the wild-typelike mutant enzyme A143Q |
650071 |
| 3.4.21.97 | A143Q/H157E |
the ratio of turnover number to Km-value is 11.7fold lower than that of the wild-typelike mutant enzyme A143Q |
650071 |
| 3.4.21.97 | A143Q/H157Q |
the ratio of turnover number to Km-value is 19fold lower than that of the wild-typelike mutant enzyme A143Q |
650071 |
| 3.4.21.97 | S134A/A143Q |
the ratio of turnover number to Km-value is 150fold lower than that of the wild-typelike mutant enzyme A143Q |
650071 |
