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Literature summary for 3.4.21.97 extracted from

  • Margosiak, S.A.; Vanderpool, D.L.; Sisson, W.; Pinko, C.; Kan, C.C.
    Dimerization of the human cytomegaolvirus protease: kinetic and biochemical characterization of the catalytic homodimer (1996), Biochemistry, 35, 5300-5307.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
citrate enhancement of activity in absence of glycerol Human betaherpesvirus 5

Protein Variants

Protein Variants Comment Organism
A134Q oligomerization at high protein concentration Human betaherpesvirus 5

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.125
-
Abz-GVVNASSRLAY(NO2)G presence of 25% glycerol, enzyme HCMV A134Q Human betaherpesvirus 5
0.134
-
Abz-GVVNASSRLAY(NO2)G presence of 50% glycerol, enzyme HCMV A134Q Human betaherpesvirus 5

Organism

Organism UniProt Comment Textmining
Human betaherpesvirus 5
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Abz-GVVNASSRLAY(NO2)G + H2O
-
Human betaherpesvirus 5 anthranilamide-GVVNA + SSRLAY(NO2)G
-
?
GVVNASCRLA + H2O
-
Human betaherpesvirus 5 GVVNA + SCRLA
-
?

Subunits

Subunits Comment Organism
dimer the catalytically active dimer is significantly dissociated above 25°C Human betaherpesvirus 5

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
activation energy below 25°C: 5340 cal/mol Human betaherpesvirus 5
40
-
dimeric enzyme is reversibly dissociated Human betaherpesvirus 5
50
-
partial denaturation Human betaherpesvirus 5

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.367
-
Abz-GVVNASSRLAY(NO2)G presence of 50% glycerol, enzyme HCMV A134Q Human betaherpesvirus 5
0.583
-
Abz-GVVNASSRLAY(NO2)G presence of 25% glycerol, enzyme HCMV A134Q Human betaherpesvirus 5