EC Number   |
Protein Variants |
Reference |
|---|
  3.2.1.B28 | A419T |
159% of wild-type activity with 4-nitrophenyl beta-D-glucoside, 109% with 4-nitrophenyl beta-D-galactoside, 53% residual activity after 1 h at 106°C |
735663 |
| 3.2.1.B28 | E372D |
200-fold reduction in specific activity |
722489 |
| 3.2.1.B28 | E372Q |
1000-fold reduction in specific activity |
722489 |
| 3.2.1.B28 | E386G |
mutation in nucleophile residue E387, mutation completely abolishes activity under statndard conditions. The addition of 2 M sodium formate as an external nucleophile leads to the recovery of 8.40% activity with accumulation of oligosaccharides. At pH 3.0 and low concentrations of sodium formate buffer, the hyperthermophilic glycosynthase shows kcat values similar to those of the wild-type and 17fold higher than those observed at the usual reactivation conditions in 2 M sodium formate at pH 6.5 |
735665 |
| 3.2.1.B28 | E417S |
mutation in phosphate binding site, 5fold increase of the efficiency of hydrolyzing o-nitrophenol-beta-D-galactopyranoside-6-phosphate. Activity on nonphosphorylated sugars is largely reduced |
326270 |
| 3.2.1.B28 | E417S/M424K/F426Y |
mutations in phosphate binding site, 3fold increase of the efficiency of hydrolyzing 2-nitrophenyl beta-D-galactopyranoside-6-phosphate. Activity on nonphosphorylated sugars is largely reduced |
326270 |
| 3.2.1.B28 | F426Y |
mutant has lower activity than the wild-type enzyme, but provides a higher ratio of transglucosylation product to hydrolysis products compared to wild-type enzyme, 2.6fold increase. The mutant enzyme has higher selectivity over the wide range of temperatures tested |
721888 |
| 3.2.1.B28 | F426Y |
mutation in phosphate binding site, results in an increased affinity for galactosides. Activity on nonphosphorylated sugars is largely reduced |
326270 |
| 3.2.1.B28 | I161V |
110% of wild-type activity with 4-nitrophenyl beta-D-glucoside, 37% with 4-nitrophenyl beta-D-galactoside, 84% residual activity after 1 h at 106°C |
735663 |
| 3.2.1.B28 | I67T |
110% of wild-type activity with 4-nitrophenyl beta-D-glucoside, 97% with 4-nitrophenyl beta-D-galactoside, 100% residual activity after 1 h at 106°C |
735663 |
