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Literature summary for 3.2.1.B28 extracted from
- Enhanced transglucosylation/hydrolysis ratio of mutants of Pyrococcus furiosus beta-glucosidase: effects of donor concentration, water content, and temperature on activity and selectivity in hexanol (2001), Biotechnol. Bioeng., 75, 656-665.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type enzyme and mutant enzymes in Escherichia coli | Pyrococcus furiosus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| F426Y | mutant has lower activity than the wild-type enzyme, but provides a higher ratio of transglucosylation product to hydrolysis products compared to wild-type enzyme, 2.6fold increase. The mutant enzyme has higher selectivity over the wide range of temperatures tested | Pyrococcus furiosus |
| M424K | mutant has lower activity than the wild-type enzyme, but provides a higher ratio of transglucosylation product to hydrolysis products compared to wild-type enzyme | Pyrococcus furiosus |
| M424K/F426Y | mutant has lower activity than the wild-type enzyme, but provides a higher ratio of transglucosylation product to hydrolysis products compared to wild-type enzyme | Pyrococcus furiosus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 21 | - |
4-nitrophenyl beta-D-glucoside | pH 5.0, 95°C, mutant enzyme F426Y | Pyrococcus furiosus |  |
| 27 | - |
4-nitrophenyl beta-D-glucoside | pH 5.0, 95°C, mutant enzyme M424K/F426Y | Pyrococcus furiosus | |
| 39 | - |
4-nitrophenyl beta-D-glucoside | pH 5.0, 95°C, mutant enzyme M424K | Pyrococcus furiosus | |
| 49 | - |
4-nitrophenyl beta-D-glucoside | pH 5.0, 95°C, wild-type enzyme | Pyrococcus furiosus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54665 | - |
x * 54665, calculated from sequence | Pyrococcus furiosus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus furiosus | Q51723 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pyrococcus furiosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-glucoside + H2O | - |
Pyrococcus furiosus | 4-nitrophenol + beta-D-glucose | - |
? | |
| additional information | the enzyme also shows transglucosylation activity | Pyrococcus furiosus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 54665, calculated from sequence | Pyrococcus furiosus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CelB | - |
Pyrococcus furiosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
assay at | Pyrococcus furiosus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.8 | - |
4-nitrophenyl beta-D-glucoside | pH 5.0, 95°C, mutant enzyme M424K/F426Y | Pyrococcus furiosus | |
| 0.9 | - |
4-nitrophenyl beta-D-glucoside | pH 5.0, 95°C, mutant enzyme F426Y | Pyrococcus furiosus | |
| 5 | - |
4-nitrophenyl beta-D-glucoside | pH 5.0, 95°C, mutant enzyme M424K | Pyrococcus furiosus | |
| 7.1 | - |
4-nitrophenyl beta-D-glucoside | pH 5.0, 95°C, wild-type enzyme | Pyrococcus furiosus | |
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