EC Number   |
Protein Variants |
Reference  |
|---|
   2.7.7.7 | L391F |
defective in the in vitro replication initiation and DNA polymerase elongation assays and fails to recognize the viral replication origin if the protein is expressed at 37°C, expression at 32°C results in activities similar to wild-type enzyme |
643641 |
| 2.7.7.7 | D190A |
retains polymerase activity, reduced exonuclease activity, changes in dependency on metal activation of exonuclease activity |
643652 |
| 2.7.7.7 | D10A |
retains polymerase activity, reduced exonuclease activity, changes in dependency on metal activation of exonuclease activity |
643653 |
| 2.7.7.7 | G418K |
increased exonuclease activity |
643660 |
| 2.7.7.7 | Q507E |
increased exonuclease activity |
643660 |
| 2.7.7.7 | Y708A |
mutation of pol delta, exhibits slow growth, sensitivity to hydroxyurea and strong mutator phenotype for frameshifts and base substitutions |
643664 |
| 2.7.7.7 | Y831A |
mutation of pol epsilon, slight sensitivity to hydroxyurea, semidominant mutator phenotype for frameshifts and base substitutions |
643664 |
| 2.7.7.7 | Y869A |
mutation of pol alpha, strain is viable, exhibits slow growth, sensitivity to hydroxyurea and spontaneous mutator phenotype for frameshifts and base substitutions |
643664 |
| 2.7.7.7 | I364Q |
binds the substrate with less efficiency than wild-type enzyme |
643672 |
| 2.7.7.7 | I364R |
unable binding of the substrate to the enzyme |
643672 |
