EC Number   |
Protein Variants |
Reference |
|---|
   2.7.7.7 | S423R |
1.6fold more effcient than wild-type, 2fold increased DNA binding affinity, pathogenic |
761958 |
| 2.7.7.7 | K70A |
19% loss of DNA polymerase activity |
726027 |
| 2.7.7.7 | Y147A |
19% loss of DNA polymerase activity |
726027 |
| 2.7.7.7 | N176A |
2% increase of DNA polymerase activity |
726027 |
| 2.7.7.7 | Y46A |
2% loss of DNA polymerase activity |
726027 |
| 2.7.7.7 | E419G |
20fold decrease in kcat/Km on dG and 670fold decrease on N2-CH2-Anth-dG, extension defect |
761958 |
| 2.7.7.7 | E128A |
23% loss of DNA polymerase activity |
726027 |
| 2.7.7.7 | T134A |
23% loss of DNA polymerase activity |
726027 |
| 2.7.7.7 | D215A |
3'-5'-exonuclease inactive mutant enzyme |
721612 |
| 2.7.7.7 | L21F |
30fold decrease in incorporation opposite N2-CH2-(9-anthracenyl)-dG (N2-CH2-Anth-dG) |
761958 |
