EC Number   |
Protein Variants |
Reference |
|---|
    2.7.7.62 | G153D |
crystal structure of mutant is determined at 2.8 A: The protein crystallizes in the hexagonal space group P31 with 4 chains in the asymmetric unit. Mutant shows reduced activity and is composed of a monomer. Results from isothermal titration calorimetry experiments show that MjCobYG153D has 10fold higher affinity for GTP than wild-type, but fails to bind the corrinoid substrate |
724319 |
| 2.7.7.62 | D83G |
in vitro activity relative to wild-type: 100%, monomer/dimer ratio: 82/18 |
-, 724319 |
| 2.7.7.62 | S100A |
in vitro activity relative to wild-type: 100%, monomer/dimer ratio: not determined |
-, 724319 |
| 2.7.7.62 | S50A |
in vitro activity relative to wild-type: 100%, monomer/dimer ratio: not determined |
724319 |
| 2.7.7.62 | T56A |
in vitro activity relative to wild-type: 102%, monomer/dimer ratio: not determined |
724319 |
| 2.7.7.62 | R13A |
in vitro activity relative to wild-type: 15%, monomer/dimer ratio: 14/86 |
724319 |
| 2.7.7.62 | K19A |
in vitro activity relative to wild-type: 16%, monomer/dimer ratio: 36/64 |
-, 724319 |
| 2.7.7.62 | G153D |
in vitro activity relative to wild-type: 2%, monomer/dimer ratio: 54/46 |
724319 |
| 2.7.7.62 | P94A |
in vitro activity relative to wild-type: 43%, monomer/dimer ratio: 52/48 |
724319 |
| 2.7.7.62 | N177R |
in vitro activity relative to wild-type: 5%, monomer/dimer ratio: not determined |
724319 |
