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Literature summary for 2.7.7.62 extracted from
- Structure and Mutational Analysis of the Archaeal GTP:AdoCbi-P Guanylyltransferase (CobY) from Methanocaldococcus jannaschii: Insights into GTP Binding and Dimerization (2011), Biochemistry, 50, 5301-5313.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Methanocaldococcus jannaschii |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| three-dimensional crystal structure of the guanylyltransferase (CobY) enzyme (mutant G153D) from Methanocaldococcus jannaschii (MjCobY)1 in complex with GTP at 2.8 A resolution is reported | Methanocaldococcus jannaschii |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D101G | in vitro activity relative to wild-type: 71%, monomer/dimer ratio: 34/66 | Methanocaldococcus jannaschii |
| D83G | in vitro activity relative to wild-type: 100%, monomer/dimer ratio: 82/18 | Methanocaldococcus jannaschii |
| E18D | in vitro activity relative to wild-type: 56%, monomer/dimer ratio: 40/60 | Methanocaldococcus jannaschii |
| G153D | crystal structure of mutant is determined at 2.8 A: The protein crystallizes in the hexagonal space group P31 with 4 chains in the asymmetric unit. Mutant shows reduced activity and is composed of a monomer. Results from isothermal titration calorimetry experiments show that MjCobYG153D has 10fold higher affinity for GTP than wild-type, but fails to bind the corrinoid substrate | Methanocaldococcus jannaschii |
| G153D | in vitro activity relative to wild-type: 2%, monomer/dimer ratio: 54/46 | Methanocaldococcus jannaschii |
| G8D | mutant non-functional, monomer/dimer ratio: 33/67 | Methanocaldococcus jannaschii |
| I144D/F146D/Y163R | monomer/dimer/aggregate ratio: 77/23/0. Specific activity (pmol/min/microgram): 156 (monomer), 49 (dimer) | Methanocaldococcus jannaschii |
| K19A | in vitro activity relative to wild-type: 16%, monomer/dimer ratio: 36/64 | Methanocaldococcus jannaschii |
| L38D/K41D | monomer/dimer/aggregate ratio: 53/25/22. Specific activity (pmol/min/microgram): 125 (monomer), 120 (dimer), 78 (aggregate) | Methanocaldococcus jannaschii |
| L38K/K41D | monomer/dimer/aggregate ratio: 52/26/22. Specific activity (pmol/min/microgram): 124 (monomer), 119 (dimer), 85 (aggregate) | Methanocaldococcus jannaschii |
| N177R | in vitro activity relative to wild-type: 5%, monomer/dimer ratio: not determined | Methanocaldococcus jannaschii |
| N179D | in vitro activity relative to wild-type: 95%, monomer/dimer ratio: not determined | Methanocaldococcus jannaschii |
| P20A | in vitro activity relative to wild-type: 93%, monomer/dimer ratio: 63/37 | Methanocaldococcus jannaschii |
| P54A | in vitro activity relative to wild-type: 62%, monomer/dimer ratio: 50/50 | Methanocaldococcus jannaschii |
| P94A | in vitro activity relative to wild-type: 43%, monomer/dimer ratio: 52/48 | Methanocaldococcus jannaschii |
| R13A | in vitro activity relative to wild-type: 15%, monomer/dimer ratio: 14/86 | Methanocaldococcus jannaschii |
| S100A | in vitro activity relative to wild-type: 100%, monomer/dimer ratio: not determined | Methanocaldococcus jannaschii |
| S50A | in vitro activity relative to wild-type: 100%, monomer/dimer ratio: not determined | Methanocaldococcus jannaschii |
| T180A | in vitro activity relative to wild-type: 73%, monomer/dimer ratio: not determined | Methanocaldococcus jannaschii |
| T53S | in vitro activity relative to wild-type: 84%, monomer/dimer ratio: 76/24 | Methanocaldococcus jannaschii |
| T56A | in vitro activity relative to wild-type: 102%, monomer/dimer ratio: not determined | Methanocaldococcus jannaschii |
| Y80F | in vitro activity relative to wild-type: 65%, monomer/dimer ratio: 74/26 | Methanocaldococcus jannaschii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + adenosylcobinamide phosphate | Methanocaldococcus jannaschii | - |
diphosphate + adenosylcobinamide-GDP | - |
? |  |
| GTP + adenosylcobinamide phosphate | Methanocaldococcus jannaschii DSM 2661 | - |
diphosphate + adenosylcobinamide-GDP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanocaldococcus jannaschii | Q58517 | - |
- |
| Methanocaldococcus jannaschii DSM 2661 | Q58517 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + adenosylcobinamide phosphate | - |
Methanocaldococcus jannaschii | diphosphate + adenosylcobinamide-GDP | - |
? | |
| GTP + adenosylcobinamide phosphate | - |
Methanocaldococcus jannaschii DSM 2661 | diphosphate + adenosylcobinamide-GDP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | MjCobYWT is comprised of a population of dimers and monomers. CobY is active in both the monomeric and dimeric forms | Methanocaldococcus jannaschii |
| monomer | MjCobYWT is comprised of a population of dimers and monomers. CobY is active in both the monomeric and dimeric forms | Methanocaldococcus jannaschii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CobY | - |
Methanocaldococcus jannaschii |
| MjCobY | - |
Methanocaldococcus jannaschii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Methanocaldococcus jannaschii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.9 | - |
assay at | Methanocaldococcus jannaschii |
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