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EC Number	Protein Variants	Commentary	Reference
2.7.7.52	C306A/C309A	the mutant does not oligo-uridylylate pre-let-7a-1 RNA in the presence of Lin28A	761973
2.7.7.52	C83F	230% of wild-type activity for transfer to terminal U residue	722967
2.7.7.52	D136A	inactive	675434
2.7.7.52	D280A	the mutation abrogates enzyme activity	762049
2.7.7.52	D297A	Km=0.1728 (UTP), kcat=0.00033/sec (UTP)	675434
2.7.7.52	D297N	Km=0.0139 (UTP), kcat=0.0000666/sec (UTP)	675434
2.7.7.52	D491A/D493A	complete loss of activity	-, 739189
2.7.7.52	D547A	catalytically dead mutant	760504
2.7.7.52	D66A	inactive	675434
2.7.7.52	D68A	inactive	675434

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Release 2023.1 (January 2023)